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Series GSE4508 Query DataSets for GSE4508
Status Public on Jun 07, 2006
Title Abundant transcripts from retrotransposons are unstable in fully-grown mouse oocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary One physiological function proposed for RNA interference (RNAi) is to constrain expression of repetitive elements and thereby reduce the incidence of retrotransposition. Consistent with this model is that inhibiting the RNAi pathway results in an increase in expression of repetitive elements in preimplantation mouse embryos. Mouse oocytes are essentially transcriptionally quiescent providing a unique opportunity to assess the stability of repetitive element-derived transcripts in these cells. We compared the transcriptome of freshly isolated fully-grown germinal vesicle (GV)-intact oocytes to that of oocytes in which meiotic maturation in vitro was inhibited for 48 h by milrinone. Consistent with the aforementioned function for RNAi is that the abundance of only a relatively small number of transcripts decreased in the cultured oocytes, when compared to changes that occur during maturation or following fertilization, and of those, several belonged to mobile elements.
Keywords: untreated GV oocytes and GV oocytes cultured in milrinone for 48h
 
Overall design untreated GV oocytes (four replicates), GV oocytes cultured in milrinome for 48h (four replicates)
 
Contributor(s) Puschendorf M, Stein P, Oakeley EJ, Schultz RM, Peters AH, Svoboda P
Citation(s) 16815300
Submission date Mar 21, 2006
Last update date Feb 11, 2019
Contact name Mareike Puschendorf
E-mail(s) mareike.puschendorf@fmi.ch
Organization name Friedrich Miescher Institute for Biomedical Research
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM101215 GV ctrl repl1
GSM101216 GV ctrl repl2
GSM101217 GV ctrl repl3
Relations
BioProject PRJNA94505

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