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Series GSE45156 Query DataSets for GSE45156
Status Public on Nov 27, 2013
Title Gene expression in alpha SMA Cre-labeled cells in periosteum during early fracture callus formation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Fracture healing is a process that involves many cell populations. In this study we characterized gene expression in a subset of cells involved in fracture healing. αSMACreERT2 mice crossed with Ai9 reporter mice that express tdTomato fluorescent protein after Cre-mediated activation were used as an experimental model. αSMA-expressing cells were labeled by tamoxifen administration, then periosteal cells from the tibia were isolated two days later (controls), or tibial fractures were performed and periosteum/soft callus tissue was collected after 2 and 6 days. The tdTomato positive cell population was isolated by flow cytometry, and subjected to microarray analysis. Histology and cell surface marker analysis indicates that αSMACreERT2 labels a mainly mesenchymal population in the periosteum that expands after fracture, and contributes to both osteogenic and chondrogenic elements of the fracture callus. We were therefore able to examine gene expression in a defined population during the early stages of fracture healing.
 
Overall design Total RNA was obtained from the tomato positive cells within the periosteal compartment of fractures from αSMACreERT2/Ai9 mice. Control animals were given 2 doses of tamoxifen, and periosteum was collected and labeled cells sorted (8-9 sex-matched mice per group). Fractures were performed after the second dose of tamoxifen, and tomato positive cells from periosteum/callus tissue were isolated 2 and 6 days after fracture (4-8 animals per sample pooled). 3 replicates for each sample are included.
 
Contributor(s) Matthews BG, Kalajzic I
Citation(s) 24190076
Submission date Mar 14, 2013
Last update date Jun 14, 2018
Contact name Brya Matthews
E-mail(s) brya.matthews@auckland.ac.nz
Organization name University of Auckland
Department Molecular Medicine and Pathology
Street address 85 Park Rd
City Grafton
State/province Auckland
ZIP/Postal code 1023
Country New Zealand
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (9)
GSM1098165 Unfractured Day 2 αSMA-labeled cells rep1
GSM1098166 Unfractured Day 2 αSMA-labeled cells rep2
GSM1098167 Unfractured Day 2 αSMA-labeled cells rep3
Relations
BioProject PRJNA193081

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45156_RAW.tar 3.1 Mb (http)(custom) TAR
GSE45156_non-normalized.txt.gz 1.5 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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