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| Status |
Public on Sep 08, 2014 |
| Title |
Reprogramming by Sall4, Nanog, Esrrb and Lin28 produces high quality iPSCs with a molecular signature of developmental potency that resembles ESCs. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To reveal a gene expression signature associated with developmental competence, we selected the following groups of iPSC lines for microarray analysis. i) “Poor quality” iPSCs: This group included the three OSKM-iPSC lines Nanog-GFP OSKM#2, Oct4-GFP OSKM#2 and KH2 OSKM27, that either did not produce fully developed pups or produced very low number of pups; ii) “Good quality” iPSCs: This group included BC_2 OSKM28 and Nanog-GFP SNEL#3, both of which gave rise to live, normal pups that survived only few hours; iii) “High quality” iPSCs consisting of Nanog-GFP SNEL#2 and Oct4-GFP SNEL#1, both of which generated live pups that survived postnatally (representative pups from each iPSC group are shown in Figure S5). iv) As controls we used Nanog-GFP, Oct4-GFP and KH229 ESCs.
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| Overall design |
Poor, good and high quality iPSC lines together with 3 embryonic stem cell lines were subjected to agilent microarray
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| Contributor(s) |
Buganim Y, Markoulaki S, Akhtar-Zaidi B, He Y, Thiru P |
| Citation(s) |
25192464 |
| Submission date |
Mar 14, 2013 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Yossi Buganim |
| E-mail(s) |
yossib@wi.mit.edu
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| Organization name |
Whitehead Institute
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| Lab |
Jaenisch
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| Street address |
9 Cambridge Center
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA193102 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45173_RAW.tar |
248.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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