 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 13, 2007 |
| Title |
Mouse Liver Regeneration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this study is to obtain an expression profile of regenerating mouse livers. We used microarray analysis to study the gene groups coordinately expressed temporally during mouse a liver regeneration. One of the syn-expression groups that showed a sharp rise in gene expression immediately after hepatectomy contains a set pancreatic exocrine genes. The syn-expression profile of pancreatic genes and the pancreatic-specific transcription factors Ptf1a and Ipf1 was confirmed using real-time PCR. Electron microscopic examination showed the presence of morphological features reminiscent of pancreatic acinar cells with the presence of characteristic zymogen granules and ER organization. These results indicate that there is a transient liver-to-pancreas transdifferentiation event at the beginning of liver regeneration. A strong coordinated up-regulation of pancreatic genes was found to accompany the injury response in the pancreas, suggesting a compensatory mechanism for pancreatic functions. There seems to be a relationship between the induction of pancreatic genes and acute phase response at the beginning of liver regeneration.
Keywords: gene expression array-based (RNA / in situ oligonucleotide)
|
| |
|
| Overall design |
Total RNA was extracted from livers of mice recovered at
various hours after partial hepatectomy (PHx 4h, 8h, 12h, 36h,
48h, 72h). We grouped livers of four to six mice together for
RNA extraction. Genes showed differentially up- or downregulated
by two-fold with respect to the normal mouse livers
were identified using GeneSpring software version 7.2 (Silicon
Genetics, Agilent Technologies).
|
| |
|
| Contributor(s) |
Tsou A, Chau Y, Chang H, Liu C, Lu Y, Lin C, Jiang J, Ke Y, Chiu J, Chi C, Chou C, Hsu M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 24, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Ann-Ping Tsou |
| E-mail(s) |
aptsou@ym.edu.tw
|
| Phone |
886-2-28267155
|
| Fax |
886-2-28264092
|
| Organization name |
National Yang-Ming University
|
| Department |
Inst. Biotechnology in Medicine
|
| Street address |
155, Li-Nong St., Sec.2
|
| City |
Taipei |
| ZIP/Postal code |
112 |
| Country |
Taiwan |
| |
|
| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
|
| Samples (14)
|
| GSM101308 |
PHx4h-2 (4h post partial hepatectomy) |
| GSM101309 |
PHx8h-1 (8h post partial hepatectomy) |
| GSM101310 |
PHx8h-2 (8h post partial hepatectomy) |
| GSM101311 |
PHx12h-1 (12h post partial hepatectomy) |
| GSM101312 |
PHx12h-2 (12h post partial hepatectomy) |
| GSM101313 |
PHx36h-1 (36h post partial hepatectomy) |
| GSM101314 |
PHx36h-2 (36h post partial hepatectomy) |
| GSM101315 |
PHx48h-1 (48h post partial hepatectomy) |
| GSM101316 |
PHx48h-2 (48h post partial hepatectomy) |
| GSM101317 |
PHx72h-1 (72h post partial hepatectomy) |
| GSM101318 |
PHx72h-2 (72h post partial hepatectomy) |
|
| Relations |
| BioProject |
PRJNA94599 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE4528_RAW.tar |
48.1 Mb |
(http)(custom) |
TAR (of CEL) |
|
|
|
|
 |
Less...
More...