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| Status |
Public on Mar 24, 2006 |
| Title |
Fast and chronic ethanol tolerance - time course |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
To better understand the molecular mechanisms underlying ethanol action we have developed an assay system to study sensitivity and fast and chronic tolerance to the sedative effects of ethanol. Flies developed fast ethanol tolerance after single exposure to 50% of ethanol vapor for 40 min. Flies can also develope chronic ethanol tolerance after 5x 40 min exposure to 50% ethanol vapor interspersed with 4x 80 min exposure to 10% ethanol vapor.
We used microarray analysis to identify genes involved in the development of fast and chronic ethanol tolerance in Drosophila. More than 600 hundred genes were found to have changed their expression level in response to different ethanol treatment. Among these genes, Homer was picked for further study and is demonstrated that its expression in ellipsoid body is critical for the normal sensitivity and fast toleance to ethanol.
Keywords: time course
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| Overall design |
Whole heads of 3 groups of Drosophila were separated for RNA extraction and hybridization on Affymetrix microarrays. These 3 groups of flies were treated with different ethanol treatment. Naive group of flies (GSM101531, GSM101532, GSM101533, GSM101534, GSM101535, GSM101536) received only 50 min of humidified air. Fast group of flies (GSM101537, GSM101538, GSM101539, GSM101540, GSM101541, GSM101542) received humidified air for 50 min followed by a single exposure of 50% ethanol vapor for 40 min. Chronic group of flies (GSM101543, GSM101544, GSM101545, GSM101546, GSM101547, GSM101548) ) received humidified air for 50 min followed by 5 exposures of 50% ethanol vapor for 40 min.
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| Contributor(s) |
Yang Z, Urizar NL, Edenberg HJ, Davis RL |
| Citation(s) |
17460067 |
| Submission date |
Mar 24, 2006 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Zhiyong Yang |
| E-mail(s) |
zhiyongyang06@yahoo.com
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| Phone |
832-326-1841
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| Organization name |
Baylor College of Medicine
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| Street address |
One Baylor Plaza
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL72 |
[DrosGenome1] Affymetrix Drosophila Genome Array |
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| Samples (18)
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| GSM101531 |
Drosophila heads_Naïve group_biological rep1_RD01D007 |
| GSM101532 |
Drosophila heads_Naïve group_biological rep2_RD01D008 |
| GSM101533 |
Drosophila heads_Naïve group_biological rep3_RD01D009 |
| GSM101534 |
Drosophila heads_Naïve group_biological rep4_RD01D010 |
| GSM101535 |
Drosophila heads_Naïve group_biological rep5_RD01D011 |
| GSM101536 |
Drosophila heads_Naïve group_biological rep6_RD01D012 |
| GSM101537 |
Drosophila heads_Fast group_biological rep1_RD01D013 |
| GSM101538 |
Drosophila heads_Fast group_biological rep2_RD01D014 |
| GSM101539 |
Drosophila heads_Fast group_biological rep3_RD01D015 |
| GSM101540 |
Drosophila heads_Fast group_biological rep4_RD01D016 |
| GSM101541 |
Drosophila heads_Fast group_biological rep5_RD01D017 |
| GSM101542 |
Drosophila heads_Fast group_biological rep6_RD01D018 |
| GSM101543 |
Drosophila heads_Chronic group_biological rep1_RD01D019 |
| GSM101544 |
Drosophila heads_Chronic group_biological rep2_RD01D020 |
| GSM101545 |
Drosophila heads_Chronic group_biological rep3_RD01D021 |
| GSM101546 |
Drosophila heads_Chronic group_biological rep4_RD01D022 |
| GSM101547 |
Drosophila heads_Chronic group_biological rep5_RD01D023 |
| GSM101548 |
Drosophila heads_Chronic group_biological rep6_RD01D024 |
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| Relations |
| BioProject |
PRJNA94603 |
| Supplementary data files not provided |
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