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| Status |
Public on Apr 30, 2006 |
| Title |
Expression data from mouse primary visual cortex under different deprivation conditions |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Two key paradigms for examining activity-dependent development of primary visual cortex (V1) involve either reduction of activity in both eyes via dark-rearing (DR) or imbalance of activity between the two eyes via monocular deprivation (MD).
Combining DNA microarray analysis with computational approaches, RT-PCR, immunohistochemistry and physiological imaging, we find that DR leads to (i) upregulation of genes subserving synaptic transmission and electrical activity, consistent with a coordinated response of cortical neurons to reduction of visual drive, and (ii) downregulation of parvalbumin, implicating parvalbumin-expressing neurons as underlying the delay in cortical maturation after DR. MD partially activates homeostatic mechanisms but differentially upregulates gene systems related to growth factors and neuronal degeneration, consistent with reorganization of connections after MD. A binding protein of Insulin-like Growth Factor 1 is highly upregulated after MD, and exogenous application of IGF1 prevents the physiological effects of MD on ocular dominance plasticity examined in vivo.
Keywords: multiple comparisons
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| Overall design |
We compared gene expression using Affymetrix microarray chips for normal reared animals, dark-reared animals and monocularly deprived animals
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| Contributor(s) |
Tropea D, Kreiman G, Sur M, Horng S, Yu H |
| Citation(s) |
16633343 |
| Submission date |
Mar 24, 2006 |
| Last update date |
Feb 18, 2018 |
| Contact name |
gabriel kreiman |
| E-mail(s) |
kreiman@mit.edu
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| Phone |
6172539547
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| URL |
http://www.mit.edu/~kreiman/
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| Organization name |
MIT
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| Department |
BCS
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| Street address |
77 Massachusetts Avenue MIT 46-5155
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (3) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
| GPL83 |
[MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array |
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| Samples (36)
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| GSM101690 |
Monocular deprivation right cortex, biological rep1 |
| GSM101691 |
Monocular deprivation right cortex, biological rep2 |
| GSM101692 |
Monocular deprivation right cortex, biological rep3 |
| GSM101693 |
Monocular deprivation left cortex, biological rep1 |
| GSM101694 |
Monocular deprivation left cortex, biological rep2 |
| GSM101695 |
Monocular deprivation left cortex, biological rep3 |
| GSM101697 |
Dark rearing, biological rep1 |
| GSM101698 |
Dark rearing, biological rep2 |
| GSM101699 |
Dark rearing, biological rep3 |
| GSM101701 |
Control, biological rep1 |
| GSM101702 |
Control, biological rep2 |
| GSM101703 |
Control, biological rep3 |
| GSM101705 |
Monocular deprivation right cortex, biological rep1 CHB MD-1 |
| GSM101706 |
Monocular deprivation right cortex, biological rep2, CHB MD-2 |
| GSM101707 |
Monocular deprivation right cortex, biological rep3, CHB MD-3 |
| GSM101708 |
Monocular deprivation left cortex, biological rep1 CHB MD-4 |
| GSM101709 |
Monocular deprivation left cortex, biological rep2 CHB MD-5 |
| GSM101710 |
Monocular deprivation left cortex, biological rep3 CHB MD-6 |
| GSM101711 |
Dark rearing, biological rep1 CHB DR-1 |
| GSM101712 |
Dark rearing, biological rep2 CHB DR-2 |
| GSM101713 |
Dark rearing, biological rep3 CHB DR-3 |
| GSM101714 |
Control, biological rep1 CHB Con-1 |
| GSM101715 |
Control, biological rep2 CHB Con-2 |
| GSM101716 |
Control, biological rep3 CHB Con-3 |
| GSM101717 |
Monocular deprivation right cortex, biological rep1 CHC MD-1 |
| GSM101718 |
Monocular deprivation right cortex, biological rep1 CHC MD-2 |
| GSM101719 |
Monocular deprivation right cortex, biological rep1 CHC MD-3 |
| GSM101720 |
Monocular deprivation right cortex, biological rep1 CHC MD-4 |
| GSM101721 |
Monocular deprivation left cortex, biological rep2 CHC MD-5 |
| GSM101722 |
Monocular deprivation left cortex, biological rep2 CHC MD-6 |
| GSM101723 |
Dark rearing, biological rep2 CHC DR-1 |
| GSM101724 |
Dark rearing, biological rep2 CHC DR-2 |
| GSM101725 |
Dark rearing, biological rep2 CHC DR-3 |
| GSM101726 |
Control, biological rep2 CHC Con-1 |
| GSM101727 |
Control, biological rep2 CHC Con-2 |
| GSM101728 |
Control, biological rep2 CHC Con-3 |
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| Relations |
| BioProject |
PRJNA94463 |
| Supplementary data files not provided |
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