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| Status |
Public on Mar 27, 2013 |
| Title |
Expression profiling in palatal mesenchymal cells stimulated with TGF-beta2 in the presence and absence of TGFbRI and Tak1 kinase inhibitors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
TGF-beta signaling in neural crest cells is required for normal craniofacial development. This signaling can be transduced via TGF-beta type I receptors (TGFbRI) using Smad-dependent or Smad independent signaling pathways.
We used microarrays to identify TGF-beta-responsive genes that are dependent either on TGFbRI kinase, Tak1 kinase or both.
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| Overall design |
Primary palatal mesenchymal cell cultures were established. Cultured cells were stimulated with TGF-beta2 in the presence or absence of TGFbRI kinase and Tak1 kinase inhibitors. Unstimulated cells were used as controls. Total RNAs were isolated and hybridized on Affymetrix microarrays.
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| Contributor(s) |
Kaartinen V, Yumote K, Thomas PS |
| Citation(s) |
23546880 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DE013085 |
The Role of TGF-Beta3 in Palatogenesis |
REGENTS OF THE UNIVERSITY OF MICHIGAN - ANN ARBOR |
VESA M KAARTINEN |
|
| Submission date |
Mar 25, 2013 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Vesa Matti Kaartinen |
| E-mail(s) |
vesak@umich.edu
|
| Phone |
734 615 4726
|
| Organization name |
University of Michigan
|
| Department |
BMS
|
| Lab |
Kaartinen
|
| Street address |
1011 N. University
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| GSM1105583 |
Untreated palatal mesenchymal cells |
| GSM1105584 |
Palatal mesenchymal cells treated with TGF-beta2 |
| GSM1105585 |
Palatal mesenchymal cells treated with SB431542 and TGF-beta2 |
| GSM1105586 |
Palatal mesenchymal cells treated with 5Z-7-Oxozeaenol and TGF-beta2 |
|
| Relations |
| BioProject |
PRJNA194531 |
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