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Series GSE45810 Query DataSets for GSE45810
Status Public on May 01, 2013
Title Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [RT-PCR]
Organism Mus musculus
Experiment type Expression profiling by RT-PCR
Summary MicroRNAs (miRNAs) are small, endogenous, non-protein coding RNAs that are an important means of post-transcriptional gene regulation. Deletion of Dicer, a key miRNA processing enzyme, is embryonic lethal in mice, and tissue-specific Dicer deletion results in developmental defects. Using a conditional knockout model, we generated mice lacking Dicer in the adrenal cortex. These Dicer knockout (KO) mice exhibited perinatal mortality and failure of the adrenal cortex during late gestation between embryonic day 16.5 (E16.5) and E18.5. Further study of Dicer KO adrenals demonstrated a significant loss of Sf1 expressing cortical cells that was histologically evident as early as E16.5 coincident with an increase in p21 and cleaved-caspase 3 staining in the cortex. However, peripheral cortical proliferation persisted in KO adrenals as assessed by anti-PCNA staining. To further characterize the embryonic adrenals from Dicer KO mice, we performed microarray analyses for both gene expression and miRNA on purified RNA isolated from control and KO adrenals of E15.5 and E16.5 embryos. Consistent with the absence of Dicer and the associated loss of miRNA-mediated mRNA degradation, we observed an up-regulation of a small subset of adrenal transcripts in Dicer KO mice, most notably the transcripts coded by the genes Nr6a1 and Acvr1c. Indeed, several miRNAs, including let-7, miR-34c, and miR-21 that are predicted to target these genes for degradation, were also markedly down-regulated in Dicer KO adrenals. Together these data suggest a role for miRNA mediated regulation of a subset of genes that are essential for normal adrenal growth and homeostasis.
 
Overall design Adrenals from control and Dicer KO litter mates were pooled separately from 4 individual litters, resulting in a total of 4 control (cre-) and 4 Dicer KO biological
 
Contributor(s) Krill KT, Gurdziel K, Heaton JH, Simon DP, Hammer GD
Citation(s) 23518926
Submission date Apr 05, 2013
Last update date Jun 04, 2013
Contact name Katherine Gurdziel
Organization name Wayne State University
Street address 275 E. Hancock
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platforms (1)
GPL16971 TaqMan OpenArray Rodent MicroRNA Panel
Samples (16)
GSM1115882 1R) e15.5 WT
GSM1115883 2R) e15.5 KO
GSM1115884 3) e15.5 WT
This SubSeries is part of SuperSeries:
GSE45812 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex
Relations
BioProject PRJNA196412

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45810_fold_change.txt.gz 6.2 Kb (ftp)(http) TXT
GSE45810_non-normalized_data.txt.gz 25.8 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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