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Series GSE4585 Query DataSets for GSE4585
Status Public on Jul 30, 2006
Title The zebrafish runzel mutant results in a novel muscular dystrophy phenotype due to a titin mutation
Organism Danio rerio
Experiment type Expression profiling by array
Summary The zebrafish runzel (ruz) carries a mutation in the titin gene resulting in muscle degeneration and embryonic lethality. At the onset of the visible phenotype (3-3.5 days post fertilization), RNA expression of the tail and trunk (primarily skeletal muscle) was compared between ruz mutants and wildtype siblings using the Affymetrix Zebrafish Gene Chip. Mutant RNA was matched with RNA of wildtype siblings from the same clutch. 3 separate clutches were used (n=3). RNA was labeled, sheered, and hybridized as in Shephard et al. (Shepard et al., 2005) and the data analyzed as in Choe et al. and Weber et al. (Choe et al., 2005; Weber et al., 2005). Results suggest a novel mechanism of titin muscular dystrophy pathogenesis including upregulation of heat shock proteins.
Keywords: disease state analysis
 
Overall design At the onset of the visible phenotype (3-3.5 days post fertilization), RNA expression of the tail and trunk (primarily skeletal muscle) was compared between ruz mutants and wildtype siblings using the Affymetrix Zebrafish Gene Chip. RNA of mutants was matched with RNA of wildtype siblings from the same clutch. 3 separate clutches were used (6 arrays total).
 
Contributor(s) Steffen LS, Guyon JR, Vogel ED, Weber GJ, Zhou Y, Zon LI, Kunkel LM
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Submission date Mar 30, 2006
Last update date Jan 25, 2018
Contact name Leta S Steffen
E-mail(s) leta_steffen@student.hms.harvard.edu
Organization name Harvard Medical School, Children's Hospital
Department Program in Genomics
Lab Louis M Kunkel
Street address 320 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM102665 Ruz 3.5dpf zebrafish muscle 1
GSM102666 Ruz 3.5dpf zebrafish muscle 2
GSM102667 Ruz 3.5dpf zebrafish muscle 3
Relations
BioProject PRJNA94433

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