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| Status |
Public on Oct 18, 2013 |
| Title |
Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B re-assembles onto the male genome during protamine-to-histone exchange. Thus, TH2B is a unique histone variant, which plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg.
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| Overall design |
Examination of TH2B binding on chromatin in meiotic (spermatocytes) and post-meiotic (round spermatids) male germ cells from adult Th2b+/tag mice (TH2B C-terminally fused to three consecutive affinity tags: His, Flag and Ha). Examination of TH2B (wild type) binding on chromatin in male germ cells from adult wt mice.
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| Contributor(s) |
Rousseaux S, Khochbin S, Montellier E, Debernardi A, Imbert J, Holota H, Bergon A, Lopez F, Gérard M, Boussouar F |
| Citation(s) |
23884607 |
| Submission date |
Apr 09, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Sophie Rousseaux |
| Organization name |
INSERM-UGA
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| Department |
IAB
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| Lab |
U1209
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| Street address |
Bd de la Chantourne
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| City |
La Tronche |
| ZIP/Postal code |
38706 |
| Country |
France |
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| Platforms (2) |
| GPL14602 |
AB SOLiD 4 System (Mus musculus) |
| GPL15907 |
AB 5500xl Genetic Analyzer (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE46137 |
Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B |
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| Relations |
| BioProject |
PRJNA196684 |
| SRA |
SRP020663 |
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