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| Status |
Public on Oct 08, 2013 |
| Title |
Differential requirement for Nfil3 during NK cell development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Nfil3 has multiple functions during conventional NK cell and thymic NK cell development. Nfil3 controls NK cell development from the earliest committed NK cell progenitor stage. Nfil3-/- prepro NK cells retained T-cell potential due to the failure to repress T cell specific genes. At later stages of NK cell development, Nfil3 regulates the formation of mature NK cells by promoting directly or indirectly the expression of transcription factor Eomes. We also demonstrate that Nfil3 is important for thymic NK cell development whereas Trail+ immature NK cells generated in the bone marrow of neonates and in the adult liver develop independent of Nfil3 function.
We used microarrays to analyse the changes in gene expression due to the presence or absence of Nfil3, both in prepro NK cells and ALP cells (all lymphoid progenitors)
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| Overall design |
Samples were obtained on two experimentation dates. Replicate 1 was processed in April 2012, replicate 2 was processed in May 2012.
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| Contributor(s) |
Seillet C, Carotta S |
| Citation missing |
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| Submission date |
Apr 10, 2013 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Victoria Core |
| E-mail(s) |
victoria@dnaarrays.org
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| Organization name |
Institute for Research in Biomedicine
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| Department |
Functional Genomics Core
|
| Street address |
Baldiri Reixac, 10
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| City |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA196725 |
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