|
| Status |
Public on Dec 31, 2013 |
| Title |
Directed differentiation of human pluripotent stem cells into cortical interneurons |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Here we demonstrate the highly efficient derivation of human cortical interneurons in a NKX2.1::GFP hESC reporter line. Manipulating the timing of SHH activation yields three distinct GFP+ populations with specific transcriptional profiles, neurotransmitter phenotypes and migratory behaviors.
|
| |
|
| Overall design |
Total RNA was isolated at day 18 of differentiation from FACS sorted NKX2.1::GFP+ cells from three varying differentiation conditions and a “No SHH” condition (no sonic hedgehog treatment) that did not contain any GFP-expressing cells (Trizol, Sigma). Samples for each group in triplicate were processed for Illumina bead arrays (Illumina HT-12) by the MSKCC genomics core facility according to the specifications of the manufacturer.
|
| |
|
| Contributor(s) |
Maroof A, Ganat Y, Studer L |
| Citation(s) |
23642365 |
| Submission date |
Apr 16, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Jeffrey Zhao |
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Genomics Core
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA197259 |
Less...
More...