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Series GSE46129 Query DataSets for GSE46129
Status Public on Aug 07, 2013
Title Transcriptome analysis of Brg1 deficient small intestinal epithelium in the context of normal and aberrant Wnt signalling
Organism Mus musculus
Experiment type Expression profiling by array
Summary Brg1 has been reported to act as a trans-activator for the Wnt pathway by interacting with beta-catenin. Given this interaction and the crucial role Wnt signalling plays in the intestinal homeostasis, we aimed to investigate the effect of Brg1 loss on gene expression in normal and Wnt activated small intestinal epithelium.
Overall design We used VillinCreERT2 Cre recombinase and loxP targeted allels of Brg1 and Apc to generate 4 cohorts of conditional knock-out mice: Cre-negative controls (n=4), Brg1 deficient (n=4), Apc deficient (n=3) and double Brg1-Apc deficient (n=4). All mice were induced by 4x80mg/kg daily injections of Tamoxifen. Epithelium enriched (gut scrapes) samples of small intestine (jejunum) were collected at day 4 post induction. Loss of Brg1 expression in the small intestinal epithelium at this time point was confirmed by immunohistochemistry.
Citation(s) 23922304
Submission date Apr 17, 2013
Last update date Jun 14, 2018
Contact name Aliaksei Holik
Organization name Cardiff University
Department School of Biosciences
Lab Prof Alan Clarke
Street address Museum Avenue
City Cardiff
ZIP/Postal code CF10 3AX
Country United Kingdom
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (15)
GSM1122263 VillinCreERT2+_Apcfl/fl_Brg1fl/fl_Rep1
GSM1122264 VillinCreERT2+_Apcfl/fl_Brg1fl/fl_Rep2
GSM1122265 VillinCreERT2+_Apcfl/fl_Brg1+/+_Rep1
BioProject PRJNA197327

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Supplementary file Size Download File type/resource
GSE46129_RAW.tar 3.1 Mb (http)(custom) TAR
Raw data included within Sample table
Processed data included within Sample table

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