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Series GSE46139 Query DataSets for GSE46139
Status Public on Oct 20, 2013
Title Genome-wide analysis of E17.5 pituitary gland gene expression of control and Insm1 mutant mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary The Insm1 gene encodes a zinc finger factor expressed in many endocrine organs. We show here that Insm1 is required for differentiation of all endocrine cell types in the pituitary. Thus, in Insm1 mutant mice, hormones characteristic of the different pituitary cell types (thyroid, follicle and melanocyte stimulating hormone, adrenocorticotrope hormone, growth hormone and prolactin) are absent or produced at markedly reduced levels. The differentiation deficit is accompanied by an up-regulated expression of components of the Notch signaling pathway. Further, skeletal muscle-specific genes are ectopically expressed, indicating that Insm1 blocks a muscle-specific expression program. Since Insm1 is also essential for differentiation of endocrine cells in the pancreas, intestine and adrenal gland, it is emerging as a transcription factor that acts in a pan-endocrine manner. The Insm1 factor contains a SNAG domain at its N-terminus, and we show here that the SNAG domain recruits histone modifying factors (Kdm1a, Hdac1/2 and Rcor1-3) and other proteins implicated in transcriptional regulation (Hmg20a/b and Gse1). Deletion of the SNAG domain in mice disrupted differentiation of pituitary endocrine cells, and resulted in an upregulated expression of components of the Notch signaling pathway and ectopic expression of skeletal muscle-specific genes. Our work demonstrates that Insm1 acts in the transcriptional network that controls differentiation of endocrine cells in the anterior pituitary gland, and requires the SNAG domain to exert this function in vivo.
Overall design Analysis of genes regulated by Insm1 in embryonic day 17.5 pituitary gland.
Total RNA from pituitary glands of E17.5 control embryos was compared to E17.5 Insm1 mutant embryos.
Contributor(s) Welcker J, Birchmeier C
Citation(s) 24227653
Submission date Apr 17, 2013
Last update date Jun 14, 2018
Contact name Jochen Welcker
Organization name MDC Berlin
Street address Robert-Rössle-Str. 10
City Berlin
ZIP/Postal code 13125
Country Germany
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM1124851 Insm1 mutant #1
GSM1124852 Insm1 mutant #2
GSM1124853 Insm1 mutant #3
BioProject PRJNA197343

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Supplementary file Size Download File type/resource
GSE46139_RAW.tar 3.1 Mb (http)(custom) TAR
GSE46139_non-normalized_data.txt.gz 2.5 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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