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Series GSE46154 Query DataSets for GSE46154
Status Public on Jul 21, 2013
Title Microarray gene expression profiling of isolated class I and class IV Drosophila dendritic arborization sensory neurons
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Class I and IV Drosophila dendritic arborization sensory neurons were isolated via magnetic bead sorting and total RNA isolated from the samples was used for gene expression profiling.
Elucidating the molecular mechanisms controlling dendrite development is key to understanding the pivotal role these structures play in influencing synaptic integration and neural function. Despite significant advances in this field, genetic pleiotropy remains a significant impediment to investigating such complex developmental processes. To circumvent this problem, we have applied class specific neuron transcriptional expression profiling coupled to an in vivo RNAi functional validation screen in order to dissect the molecular bases of Drosophila class I and class IV dendritic arborization (da) neuron dendritogenesis.
 
Overall design Gene expression profiling of class I and IV da neurons, isolated via magnetic bead sorting (using protocols as described in Iyer et al., 2009), was performed at the third instar larval stage of development from independent cell isolations from 40-50 age-matched third instar larvae expressing mCD8::GFP under the control of either GAL4ppk.1.9 driver or Gal80ppk; GAL4221. For controls, age matched whole larval homogenate RNA was used.
 
Contributor(s) Iyer EP, Iyer SC, Cox DN
Citation(s) 23977298, 30395636
Submission date Apr 17, 2013
Last update date Nov 30, 2018
Contact name Daniel N Cox
E-mail(s) dcox5@gmu.edu, eramacha@masonlive.gmu.edu
Phone 703-993-4730
Organization name George Mason University
Department School of Systems Biology, Krasnow Institute
Lab Cox Lab
Street address 4400 University Drive, MSN 2A1
City Fairfax
State/province VA
ZIP/Postal code 22030
Country USA
 
Platforms (1)
GPL7300 Agilent-018972 D. melanogaster (FruitFly) Oligo Microarray 4x44K G2519F (Probe Name version)
Samples (7)
GSM1125131 Class-I da neuron replicate-1
GSM1125132 Class-I da neuron replicate-2
GSM1125133 Class-IV da neuron replicate-1
Relations
Affiliated with GSE69353
BioProject PRJNA197378

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46154_RAW.tar 54.5 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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