|
| Status |
Public on May 01, 2014 |
| Title |
The spatio-temporal program of DNA replication is driven by specific epigenetic signatures in human cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We report the application of single-molecule-based sequencing technology for high-throughput profiling of DNA replication origins in K562 mammalian cells. We have optimized a specific method of peak detection adapted to the signal produced by the sequencing of short nascent strands (SNS) that are specific of replication initiation, with the aim to have both a good sensitivity and specificity. We demonstrated the existence of the spatiao-temporal program of DNA replication driven by a specific epigenetic signature.
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| |
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| Overall design |
K562 human cells; five samples subjected to SNS-Seq.
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| |
|
| Contributor(s) |
Picard F, Cadoret J, Audit B, Arneodo A, Poulain J, Duret L, Prioleau M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 18, 2013 |
| Last update date |
Oct 17, 2019 |
| Contact name |
Jean-Charles Cadoret |
| E-mail(s) |
jean-charles.cadoret@ijm.fr
|
| Organization name |
CNRS/ Université de Paris
|
| Street address |
15 rue hélène Brion
|
| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
| |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (5)
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|
| Relations |
| BioProject |
PRJNA197531 |
| SRA |
SRP021179 |
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