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Series GSE46211 Query DataSets for GSE46211
Status Public on Oct 01, 2013
Title Gene expression profiling of anterior and posterior palatal tissue from Tgfbr2 mutant mouse models
Organism Mus musculus
Experiment type Expression profiling by array
Summary The overall goal of this project is to investigate the role of TGF-beta signaling in epithelial cells as it pertains to the orientation of muscle fibers in the soft palate during embryogenesis. Here, we first conducted gene expression profiling of the anterior and posterior portions of the palate from wild-type mice. In addition, we also conducted gene expression profiling of the posterior palate in mutant mice with an epithelium-specific conditional inactivation of the Tgfbr2 gene. The latter mice provide a model of submucosal cleft palate, which is a congenital birth defect commonly observed in many syndromic conditions.
 
Overall design To investigate the adverse effects of dysfunctional TGF-Beta signaling on tissue-tissue interaction between the palatal epithelium and myofibers during palatogenesis, we analyzed mice with an epithelial cell-specific conditional inactivation of Tgfbr2 (Tgfbr2fl/fl;K14-Cre). We performed microarray analyses of anterior palatal tissue and posterior palatal tissue of E15.5 Tgfbr2fl/fl control mice (n=5, each region), and posterior palatal tissue of Tgfbr2fl/fl;K14-Cre mutant mice, collected at embryonic day 15.5 (n=5). Control samples and mutant samples are from separate litters.
 
Contributor(s) Iwata J, Suzuki A, Pelikan RC, Chai Y
Citation(s) 24496627
Submission date Apr 19, 2013
Last update date Feb 11, 2019
Contact name Richard Pelikan
Organization name Oklahoma Medical Research Foundation
Department Genes & Human Disease Research Program
Lab Gaffney Lab
Street address 825 NE 13th St
City Oklahoma City
State/province Oklahoma
ZIP/Postal code 73104
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM1126414 Tgfbr2 fl/fl Control Anterior Palate 1
GSM1126415 Tgfbr2 fl/fl Control Anterior Palate 2
GSM1126416 Tgfbr2 fl/fl Control Anterior Palate 3
Relations
BioProject PRJNA198069

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46211_RAW.tar 64.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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