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| Status |
Public on Aug 08, 2013 |
| Title |
Expression analysis of Prdm4 mutant and overexpressing mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Expression profiling of wild-type, Prdm4 null and Prdm4-EGFP overexpressing mouse embryonic stem cells using Illumina whole genome V2 arrays.
The hypothesis tested was that Prdm4 regulates gene expression consistant with Prdm4 genomic binding identified by ChIP-seq
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| Overall design |
Prdm4 null embryonic stem cells were generated by gene targeting. Prdm4-EGFP overexpressing cells were generated through stable transfection. Total RNA was obtained from six wild-type ES cell clones, four Prdm4 null mutant ES cell clones and six Prdm4-EGFP overexpressing ES cell clones and hybridized to Illumina WG6_V2 arrays
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| |
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| Contributor(s) |
Bogani D, Morgan M, Nelson A, McGouran J, Kessler B, Bikoff E, Robertson E |
| Citation(s) |
23918801 |
| Submission date |
Apr 23, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Andrew Christopher Nelson |
| E-mail(s) |
a.nelson.1@warwick.ac.uk
|
| Organization name |
University of Warwick
|
| Department |
School of Life Sciences
|
| Street address |
Gibbet Hill Campus
|
| City |
Coventry |
| ZIP/Postal code |
CV4 7AL |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (16)
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| Relations |
| BioProject |
PRJNA198721 |
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