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Series GSE46361 Query DataSets for GSE46361
Status Public on Apr 28, 2015
Title Transcription of Tnfaip3 is regulated by NF-kB and p38 via C/EBPβ in activated macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary Macrophages play a pivotal role in the immune system through recognition and elimination of microbial pathogens. Toll-like receptors (TLRs) on macrophages interact with microbial substances and initiate signal transduction through intracellular adapters. TLR4, which is important for the response to lipopolysaccharide (LPS), triggers downstream signaling mediators and eventually activates IkB kinase (IKK) complex and mitogen-activated protein kinases (MAPKs) such as p38. Previous reports revealed that, in addition to NF-kB, the induction of some LPS-inducible genes in macrophages required another transcription factor whose activity depends on p38. However, these transcription factors remained to be identified.
Among these genes, NF-kB and C/EBPβ, a p38 downstream transcription factor, were predicted to co-regulate genes in LPS-stimulated BMDMs. Based on the subsequent results of a chromatin immunoprecipitation assay, we demonstrated that Tnfaip3 is regulated by both NF-kB and p38-dependent C/EBPβ. These results elucidate our understanding of the tight regulation of innate immunity.
Overall design In order to identify p38-activated transcription factors that cooperate with NF-kB in response to LPS stimulation, microarrays were used to identify genes regulated by both NF-kB and p38 using wild-type, IKK-depleted, and p38 inhibitor-treated mouse bone marrow-derived macrophages (BMDMs). In silico analysis of transcription factor binding sites was used to predict the potential synergistic transcription factors from the co-expressed genes.
Contributor(s) Lai L, Lai T, Wu S, Tsai M, Hsu L
Citation(s) 24023826
Submission date Apr 24, 2013
Last update date Jun 14, 2018
Contact name Mong-Hsun Tsai
Phone +886-2-3366-6009
Organization name National Taiwan University
Department Institute of Biotechnology
Street address 4F, No. 81, Chang-Xing St.
City Taipei
ZIP/Postal code 106
Country Taiwan
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (24)
GSM1129160 WT_0h_rep1
GSM1129161 WT_4h_rep1
GSM1129162 IKK_KO_0h_rep1
BioProject PRJNA203653

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46361_RAW.tar 3.1 Mb (http)(custom) TAR
GSE46361_non-normalized.txt.gz 3.7 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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