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| Status |
Public on Apr 28, 2015 |
| Title |
Transcription of Tnfaip3 is regulated by NF-kB and p38 via C/EBPβ in activated macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Macrophages play a pivotal role in the immune system through recognition and elimination of microbial pathogens. Toll-like receptors (TLRs) on macrophages interact with microbial substances and initiate signal transduction through intracellular adapters. TLR4, which is important for the response to lipopolysaccharide (LPS), triggers downstream signaling mediators and eventually activates IkB kinase (IKK) complex and mitogen-activated protein kinases (MAPKs) such as p38. Previous reports revealed that, in addition to NF-kB, the induction of some LPS-inducible genes in macrophages required another transcription factor whose activity depends on p38. However, these transcription factors remained to be identified.
Among these genes, NF-kB and C/EBPβ, a p38 downstream transcription factor, were predicted to co-regulate genes in LPS-stimulated BMDMs. Based on the subsequent results of a chromatin immunoprecipitation assay, we demonstrated that Tnfaip3 is regulated by both NF-kB and p38-dependent C/EBPβ. These results elucidate our understanding of the tight regulation of innate immunity.
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| Overall design |
In order to identify p38-activated transcription factors that cooperate with NF-kB in response to LPS stimulation, microarrays were used to identify genes regulated by both NF-kB and p38 using wild-type, IKK-depleted, and p38 inhibitor-treated mouse bone marrow-derived macrophages (BMDMs). In silico analysis of transcription factor binding sites was used to predict the potential synergistic transcription factors from the co-expressed genes.
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| Contributor(s) |
Lai L, Lai T, Wu S, Tsai M, Hsu L |
| Citation(s) |
24023826 |
| Submission date |
Apr 24, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Mong-Hsun Tsai |
| E-mail(s) |
motiont@gmail.com
|
| Phone |
+886-2-3366-6009
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| Organization name |
National Taiwan University
|
| Department |
Institute of Biotechnology
|
| Street address |
4F, No. 81, Chang-Xing St.
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| City |
Taipei |
| ZIP/Postal code |
106 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA203653 |
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