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Series GSE46371 Query DataSets for GSE46371
Status Public on Sep 01, 2013
Title Expression data from zebrafish (Danio rerio) embryos exposed to methyl tert-butyl ether
Organism Danio rerio
Experiment type Expression profiling by array
Summary Methyl tert-butyl ether (MTBE) has been shown to target developing vasculature in piscine and mammalian model systems. In the zebrafish, MTBE induces vascular lesions throughout development. These lesions result from exposure to MTBE at an early stage in development (6-somites to Prim-5 stages). During this time period, transcript levels of vegfa, vegfc, and vegfr1 were significantly decreased in embryos exposed to 5 mM MTBE.
We performed global gene analysis as an unbiased approach to discover possible modes of action of MTBE vascular toxicity.
Overall design Embryos were exposed at 3 hours post fertilization (hpf) in triplicate to one of three concentrations of MTBE: 5mM (induces vascular lesions and significantly decreases vegfa), 0.625mM (NOAEL; no observed adverse effect level), and 0.00625mM (100-fold below NOAEL), or to embryo media (control). Samples were collected at 6-somites (~15hpf), 21-somites (~24 hpf), and Prim-5 (~30 hpf) stages of development. Embryos were meticulously staged at exposure and at the time of collection to maintain a homogeneous population. Our experimental design sought to explore the effect of three concentrations MTBE on three different stages of zebrafish embryonic development during the critical period established for the chemical. This time period also corresponds to an important time in the cardiovascular system develop of our model vertebrate.
Contributor(s) Bonventre JA, Kung TS, White LA, Cooper KR
Citation(s) 24128854
Submission date Apr 25, 2013
Last update date Jan 25, 2018
Contact name Josephine Ann Bonventre
Organization name Oregon State University
Department Environmental & Molecular Toxicology
Lab Harper
Street address 1011 Agriculture & Life Sciences Building
City Corvallis
State/province OR
ZIP/Postal code 97331
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (36)
GSM1129268 6-somites_Control, bio rep 1
GSM1129269 6-somites_Control, bio rep 2
GSM1129270 6-somites_Control, bio rep 3
BioProject PRJNA198857

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46371_RAW.tar 61.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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