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| Status |
Public on Apr 26, 2013 |
| Title |
Direct conversion of fibroblasts to neurons by a new mechanism – p53 regulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Direct conversion from fibroblast to neuron has recently been successfully induced bypassing the pluripotent state. However, the conversion takes a few months with low percentages of success. Here we found that depletion of p53, which can converted fibroblasts into three major neural lineages: neurons, astrocytes and oligodendrocytes. Furthermore, our method provided a high efficiency of conversion in aging fibroblasts, where published methods failed. This finding may help developing a prototype for neuron replacement therapy, including foraging people vulnerable to neurological disorders.
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| Overall design |
p53 has been shown to inhibit reprogramming of fibroblasts to iPS cells, by depletion of p53 in human fibroblasts, we study the function of p53 in induced neuron process. By induction of p53 knockdown fibroblasts with special neuron medium, we can get mature neurons directly. In the induction process, many neurogenic transcription factors were up-regulated, and we prove that p21 is not involved in this process.
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| Contributor(s) |
Zhou D, Zhang Z, He L, Du J, Zhang F, Sun C, Zhou Y, Lin G, Song K, Wu L, Yang Q |
| Citation(s) |
24934763 |
| Submission date |
Apr 25, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
chongkui sun |
| E-mail(s) |
sunchk12@gmail.com
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| Phone |
+8602885503484
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| Organization name |
Sichuan University
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| Department |
State key laboratory of oral diseases
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| Lab |
cancer biology division
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| Street address |
No.14, Sec. 3, Renmin Road South
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| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA199749 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE46392_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE46392_non-normalized.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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