|
| Status |
Public on May 30, 2013 |
| Title |
Bivalent Chromatin Marks Developmental Regulatory Genes in the Mouse Embryonic Germline in Vivo |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We developed a ChIP protocol for the analysis of histone marks using less than 10,000 cells per IP, and used it to investigate the chromatin state of E11.5 mouse primordial germ cells (PGCs). A genome-wide ChIP-Seq analysis of E11.5 PGCs revealed a distribution of H3K4me3/H3K27me3 bivalent domains highly enriched for developmental regulatory genes.
|
| |
|
| Overall design |
H3K4me3 and H3K27me3 ChIP-Seq from mouse E11.5 primordial germ cells.
|
| |
|
| Contributor(s) |
Sachs M, Onodera CS, Blaschke K, Ebata KT, Song JS, Ramalho-Santos M |
| Citation(s) |
23727241 |
| Submission date |
Apr 25, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Courtney Onodera |
| E-mail(s) |
courtney.onodera@ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Street address |
1450 Third St, HD374
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA199926 |
| SRA |
SRP021522 |
