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Series GSE46410 Query DataSets for GSE46410
Status Public on Nov 05, 2013
Title Differential gene expression in mouse kidney following 72 hours of colistin exposure by Illumina WG-6_V2 array
Organism Mus musculus
Experiment type Expression profiling by array
Summary Colistin sulfate (polymixin E) is an antibiotic prescribed with resurging frequency for multidrug resistant gram negative bacterial infections. It is associated with nephrotoxicity in humans in up to 33% of cases. Little is known regarding genes involved in colistin nephrotoxicity. A murine model of colistin-mediated kidney injury was developed. C57/BL6 mice were administered saline or colistin at a dose of 16mg/kg/day in 2 divided doses. An Illumina gene expression array was performed on kidney RNA harvested 72 hours after first colistin dose to identify differentially expressed genes early in drug treatment. Array platform was MouseWG-6, 48,000 probes. Drug given intraperitoneal.
 
Overall design Total RNA was isolated from mouse kidneys which were harvested upon sacrifice. 12 total 10 week old C57 bl6 mice were analyzed. Six mice were administered 0.1% saline twice daily, six mice were given colistin at 16 mg/kg/day in 2 divided doses. The 2 groups were randomized to 2 different MouseWG-6_V2_0_R2_11278593 and groups were compared.
 
Contributor(s) Eadon MT, Cunningham PN, Bao R, Hack BK
Citation(s) 23922129
Submission date Apr 26, 2013
Last update date Jan 16, 2019
Contact name Michael Thomas Eadon
Organization name Indiana University
Department Medicine
Street address 950 W. Walnut St R2 202
City Indianapolis
State/province IN
ZIP/Postal code 46202
Country USA
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (12)
GSM1129870 Kidney_saline_1
GSM1129871 Kidney_colistin_4
GSM1129872 Kidney_saline_2
Relations
BioProject PRJNA200297

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46410_RAW.tar 15.8 Mb (http)(custom) TAR
GSE46410_non_normalized.txt.gz 4.7 Mb (ftp)(http) TXT
GSE46410_non_normalized_gene_level.txt.gz 3.4 Mb (ftp)(http) TXT
GSE46410_normalized_gene_level.txt.gz 3.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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