|
| Status |
Public on Aug 05, 2013 |
| Title |
Small RNA sequencing of human preovulatory cumulus and mural granulosa cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signalling and are involved in the processes of folliculogenesis and oocyte maturation. Studies on gene expression and regulation in human granulosa cells are of interest due to their potential for estimating the oocyte viability and IVF success. However, the post-transcriptional gene expression studies on miRNA level in the human ovary have been scarce. The current study determined the miRNA profile by deep sequencing of the two intrafollicular somatic cell types: mural and cumulus granulosa cells isolated from women undergoing controlled ovarian stimulation and in vitro fertilization.
|
| |
|
| Overall design |
Paired cumulus and mural granulosa samples were analysed from 3 women participating in IVF procedure. Libraries of all 6 samples were sequenced twice, generating 2 technical replicates for each sample. Differential gene expression study was performed on the pooled results of technical replicates.
|
| |
|
| Contributor(s) |
Velthut-Meikas A, Simm J |
| Citation(s) |
23660593 |
| Submission date |
Apr 29, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Agne Velthut-Meikas |
| Organization name |
Tallinn University of Technology
|
| Department |
Department of Chemistry and Biotechnology
|
| Street address |
Akadeemia tee 15
|
| City |
Tallinn |
| ZIP/Postal code |
12618 |
| Country |
Estonia |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE46508 |
Small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes |
|
| Relations |
| BioProject |
PRJNA200699 |
| SRA |
SRP021911 |
Less...
More...