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| Status |
Public on Nov 04, 2013 |
| Title |
Transcriptome signature of white adipose tissue, liver, and skeletal muscle in 24 hours fasted mice (C57Bl/6J) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Fasting is the process of metabolic adaption to food deprivation that is taking place in most organisms, e.g. during the daily resting phase in mammals. Furthermore, in biomedical research fasting is used in most metabolic studies to synchronize nutritional states of study subjects. Because there is a lack of standardization for this procedure, we need a deeper understanding of the dynamics and the molecular players in fasting. In this study we investigated the transcriptome signature of white adipose tissue, liver, and skeletal muscle in 24 hours fasted mice (and chow fat controls) using Affymetrix whole-genome microarrays.
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| Overall design |
Food was withdrawn from the fasting group at the beginning of the light phase (9 a.m.) when mice are in their inactive phase. Mice were sacrificed 24 hours later by cervical dislocation. Chow-fed controls had ad libitium access to food during this time. Edidymal white adipose tissue, liver, and skeletal muscle were dissected out, shock frozen in liquid nitrogen and stored at -80°C.
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| Contributor(s) |
Prokesch A, Schupp M, Chen F, Briggs ER, Rao S, Bogner-Strauss JG, Lazar MA, Baldwin D, Pessentheiner AR, Pelzmann HJ |
| Citation(s) |
24191950 |
| Submission date |
Apr 30, 2013 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Andreas Prokesch |
| E-mail(s) |
andreas.prokesch@medunigraz.at
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| Organization name |
Medical University Graz
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| Department |
Institute of Cell Biology, Histology, and Embryology
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| Street address |
Harrachgasse 21/7
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| City |
Graz |
| State/province |
Styria |
| ZIP/Postal code |
8010 |
| Country |
Austria |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (30)
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| GSM1131278 |
ad libitum chow, 24h, white adipose tissue, biological replicate 1 |
| GSM1131279 |
ad libitum chow, 24h, white adipose tissue, biological replicate 2 |
| GSM1131280 |
ad libitum chow, 24h, white adipose tissue, biological replicate 3 |
| GSM1131281 |
ad libitum chow, 24h, white adipose tissue, biological replicate 4 |
| GSM1131282 |
ad libitum chow, 24h, white adipose tissue, biological replicate 5 |
| GSM1131283 |
ad libitum chow, 24h, liver, biological replicate 1 |
| GSM1131284 |
ad libitum chow, 24h, liver, biological replicate 2 |
| GSM1131285 |
ad libitum chow, 24h, liver, biological replicate 3 |
| GSM1131286 |
ad libitum chow, 24h, liver, biological replicate 4 |
| GSM1131287 |
ad libitum chow, 24h, liver, biological replicate 5 |
| GSM1131288 |
ad libitum chow, 24h, skeletal muscle, biological replicate 1 |
| GSM1131289 |
ad libitum chow, 24h, skeletal muscle, biological replicate 2 |
| GSM1131290 |
ad libitum chow, 24h, skeletal muscle, biological replicate 3 |
| GSM1131291 |
ad libitum chow, 24h, skeletal muscle, biological replicate 4 |
| GSM1131292 |
ad libitum chow, 24h, skeletal muscle, biological replicate 5 |
| GSM1131293 |
fasted, 24h, white adipose tissue, biological replicate 1 |
| GSM1131294 |
fasted, 24h, white adipose tissue, biological replicate 2 |
| GSM1131295 |
fasted, 24h, white adipose tissue, biological replicate 3 |
| GSM1131296 |
fasted, 24h, white adipose tissue, biological replicate 4 |
| GSM1131297 |
fasted, 24h, white adipose tissue, biological replicate 5 |
| GSM1131298 |
fasted, 24h, liver, biological replicate 1 |
| GSM1131299 |
fasted, 24h, liver, biological replicate 2 |
| GSM1131300 |
fasted, 24h, liver, biological replicate 3 |
| GSM1131301 |
fasted, 24h, liver, biological replicate 4 |
| GSM1131302 |
fasted, 24h, liver, biological replicate 5 |
| GSM1131303 |
fasted, 24h, skeletal muscle, biological replicate 1 |
| GSM1131304 |
fasted, 24h, skeletal muscle, biological replicate 2 |
| GSM1131305 |
fasted, 24h, skeletal muscle, biological replicate 3 |
| GSM1131306 |
fasted, 24h, skeletal muscle, biological replicate 4 |
| GSM1131307 |
fasted, 24h, skeletal muscle, biological replicate 5 |
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| Relations |
| BioProject |
PRJNA200652 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE46495_RAW.tar |
133.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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