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| Status |
Public on Apr 13, 2006 |
| Title |
Genes responsive to bisphenol A in testicular Sertoli cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We performed global scale microarray analysis to identify detailed mechanisms by which bisphenol A (BPA) induce cell death by using an Affymetrix GeneChip system. Testicular Sertoli TTE3 cells used in the present study were derived from transgenic mice harboring a temperature-sensitive simian virus 40 large T-antigen. Cell death accompanying endoplasmic reticulum stress was observed in the cells treated with 0.2 mM BPA. Of the 22,690 probe sets analyzed, approximately 1,300 genes were down- and up-regulated by a factor of 2.0 or greater in the cells treated with BPA.
Keywords: bisphenol A, gene expression, testicular Sertoli cell
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| Overall design |
TTE3 cells were treated with BPA for 0, 3, 6, and 12 h. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with the Mouse Expression Array 430A which was spotted with 22,690 probe sets. Sample preparation for array hybridization was carried out as described in the manufacture’s instructions.
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| Web link |
http://www.lsrc.u-toyama.ac.jp/mgrc/html/microarray.html
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| Contributor(s) |
Tabuchi Y, Takasaki I |
| Citation(s) |
16713995 |
| Submission date |
Apr 11, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Yoshiaki Tabuchi |
| E-mail(s) |
ytabu@cts.u-toyama.ac.jp
|
| Organization name |
University of Toyama
|
| Department |
Life Science Research Center
|
| Lab |
Division of Molecular Genetics Research
|
| Street address |
2630 Sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (8)
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| GSM104743 |
TTE3 cells treated with BPA for 0 h-1 |
| GSM104744 |
TTE3 cells treated with BPA for 0 h-2 |
| GSM104745 |
TTE3 cells treated with BPA for 3 h-1 |
| GSM104746 |
TTE3 cells treated with BPA for 3 h-2 |
| GSM104747 |
TTE3 cells treated with BPA for 6 h-1 |
| GSM104748 |
TTE3 cells treated with BPA for 6 h-2 |
| GSM104749 |
TTE3 cells treated with BPA for 12 h-1 |
| GSM104750 |
TTE3 cells treated with BPA for 12 h-2 |
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| Relations |
| BioProject |
PRJNA95289 |
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