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| Status |
Public on May 01, 2013 |
| Title |
Molecular profile of parvalbumin-immunoreactive neurons in superior temporal cortex in schizophrenia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Dysregulation of pyramidal cell network function by the soma- and axon-targeting inhibitory neurons that contain the calcium-binding protein parvalbumin (PV) represents a core pathophysiological feature of schizophrenia. In order to gain insight into the molecular basis of their functional impairment, we used laser capture microdissection (LCM) to isolate PV-immunolabeled neurons from layer 3 of Brodmann’s area 42 of the superior temporal gyrus (STG) from postmortem schizophrenia and normal control brains. We then extracted ribonucleic acid (RNA) from these neurons and determined their messenger RNA (mRNA) expression profile using the Affymetrix platform of microarray technology. 739 mRNA transcripts were found to be differentially expressed in PV neurons in subjects with schizophrenia, including genes associated with WNT (wingless-type), NOTCH and PGE2 (prostaglandin E2) signaling, in addition to genes that regulate cell cycle and apoptosis. Of these 739 genes, only 89 (12%) were also differentially expressed in pyramidal neurons as found in the accompanying study, suggesting that the molecular pathophysiology of schizophrenia appears to be predominantly neuronal type-specific. Taken together, findings of this study provide a neurobiological framework within which hypotheses of the molecular mechanisms that underlie the dysfunction of PV neurons in schizophrenia can be generated and experimentally explored and, as such, may ultimately inform the conceptualization of targeted molecular intervention.
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| Overall design |
Gene expression microarray from mRNA isolated from parvalbumin cells in layer 3 of the STG from 8 normal controls and 8 subjects with schizophrenia. There was no significant difference between diagnosis groups for age, sex, and post mortem interval (PMI).
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| Contributor(s) |
Pietersen CY, Mauney SA, Kim SS, Passeri E, Lim MP, Goldstein JM, Petreyshen TL, Seidman LJ, Shenton ME, McCarley RW, Sonntag K, Woo TW |
| Citation missing |
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| Submission date |
Apr 30, 2013 |
| Last update date |
May 02, 2013 |
| Contact name |
Sarah Mauney |
| E-mail(s) |
smauney@mclean.harvard.edu
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| Phone |
6178552079
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| Fax |
6178553479
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| Organization name |
McLean Hospital
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| Lab |
Laboratory for Cellular Neuropathology
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| Street address |
115 Mill St.
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| City |
Belmont |
| State/province |
MA |
| ZIP/Postal code |
02478 |
| Country |
USA |
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| Platforms (1) |
| GPL1352 |
[U133_X3P] Affymetrix Human X3P Array |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA200703 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE46509_RAW.tar |
74.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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