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Series GSE4656 Query DataSets for GSE4656
Status Public on Mar 11, 2007
Title day3 brown fat from wt compared to day3 from Gyk ko
Organism Mus musculus
Experiment type Expression profiling by array
Summary Glycerol kinase (GK) is at the interface of fat and carbohydrate metabolism and has been implicated in insulin resistance and type 2 diabetes mellitus (T2DM). To define GK’s role in insulin resistance, we examined gene expression in brown adipose tissue in a glycerol kinase (Gyk) knockout (KO) mouse model using microarray analysis. Global gene expression profiles of KO mice were distinct from wild type (WT) with 668 genes that were differentially expressed. These included genes involved in lipid metabolism, carbohydrate metabolism, insulin signaling, and insulin resistance. Real-Time (RT) PCR analysis confirmed the differential expression of selected genes involved in lipid and carbohydrate metabolism. PathwayAssist analysis confirmed direct and indirect connections between GK and genes in lipid metabolism, carbohydrate metabolism, insulin signaling, and insulin resistance. Network Component Analysis (NCA) showed that the transcription factors, peroxisome proliferator-activated receptor gamma (PPAR-γ), sterol regulatory element binding factor 1 (SREBP-1), SREBP-2, signal transducer and activator of transcription 3 (STAT3), STAT5, trans-acting transcription factor 1 (SP1), CCAAT/enhancer binding protein alpha (CEBP-α), cAMP responsive element binding protein 1 (CREB), glucocorticoid receptor (GR), and PPAR-α have altered activity in the KO mice. NCA also revealed the individual contribution of these transcription factors on the expression of genes altered in the microarray data. This study elucidates the transcription network of Gyk and further confirms a role for Gyk, a simple Mendelian disorder, in insulin resistance and T2DM, a common complex genetic disorder.
Keywords: genotype state analysis
Overall design 7 samples are analyzed; 3 wildtype and 4 KO. the WT samples are used as control and KO samples are used as the experimental group.
Citation(s) 17406644
Submission date Apr 11, 2006
Last update date Feb 11, 2019
Contact name Lola Rahib
Phone 310-825-7967
Fax 310-794-5446
Organization name UCLA
Department Human Genetics
Lab Katrina Dipple
Street address 695 Charles E young Drive south Gonda 5335
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (7)
GSM104736 brownfat_KO1_day3
GSM104738 brownfat_WT1_day3
GSM104740 brownfat_KO2_day3
BioProject PRJNA95301

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