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Status |
Public on May 30, 2006 |
Title |
Changes in gene expression when Nab3 is non-functional. |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by array
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Summary |
Nrd1 and Nab3 are two yeast RNA binding proteins which have been shown to be involved in transcription termination of non poly(A) genes. We have used expression profiling of a Nab3 mutant to discover novel RNA targets of the Nrd1 and Nab3 transcription termination pathway. Failure to terminate RNA polymerase II by Nab3 leads to continued transcription well beyond the correct termination sites, altering the expression of adjacent downstream genes. Using this concept, our microarray uncovered the up-regulation of numerous genes that are located downstream of “cryptic unstable transcripts”, transcripts that are transcribed, terminated and rapidly degraded by Nrd1, Nab3, and the nuclear exosome. Keywords: yeast temperature sensitive mutant 25C vs 37C
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Overall design |
Four yeast total RNA samples where analyzed in this study. These RNAs come from two separate strains of yeast, each strain at either the permissive temperature (25C) or the non-permissive temperature (37C). One of these strains is a wild type strain of yeast used as a control. The other strain has a mutation in the Nab3 protein that confers temperature sensitivity at the non-permissive temperature of 37C. After two hours at the non-permissive temperature, we observe disruptions in the Nrd1/Nab3 transcription termination pathway and this is the scheme that we followed for our microarray experiment. Our goal was to observe the global expression changes after 2 hours without Nab3 function.
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Contributor(s) |
Arigo JT, Corden JL |
Citation(s) |
16973436 |
Submission date |
Apr 11, 2006 |
Last update date |
Jul 01, 2016 |
Contact name |
Jeff L Corden |
Organization name |
Johns Hopkins School of Medicine
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Department |
Molecular Biology and Genetics
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Street address |
725 N. Wolfe St.
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City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21202 |
Country |
USA |
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Platforms (1) |
GPL90 |
[YG_S98] Affymetrix Yeast Genome S98 Array |
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Samples (4)
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Relations |
BioProject |
PRJNA95303 |