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Series GSE46695 Query DataSets for GSE46695
Status Public on Sep 01, 2013
Title Inhibition of astroglial NF-kB regulates oligogenesis following spinal cord injury.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Astrocytes are taking the center stage in neurotrauma and neurological disease as they appear to play a dominant role in the inflammatory processes associated with these conditions. Previously, we reported that inhibiting nuclear factor kappa B (NF-kB) activation in astrocytes, by using a transgenic mouse model (GFAP-IκBα-dn mice), results in improved functional recovery following spinal cord injury (SCI), with increased white matter preservation and axonal sparing. In the present study we sought to determine whether this improvement, due to inhibiting NF-k-B activation in astrocytes, could be the result of enhanced oligogenesis in our GFAP-IκBα-dn mice. To gain insight into the underlying molecular mechanisms, we performed microarray analysis in naïve and 3 days, 3 and 6 weeks following SCI in GFAP-IκBα-dn and wild type (WT) littermate mice. Surprisingly, we found the largest number of genes differentially regulated between GFAP-IκBα-dn and WT mice 6 weeks post-injury. Interestingly, the data suggested that inhibiting astroglial NF-kB alters the inflammatory environment to support oligogenesis. Furthermore, confirmation of microarray data with qPCR and western blotting analysis and using BrdU labeling along with cell specific immunohistochemistry, confocal microscopy and quantitative cell counts, we demonstrate a significant increase in oligogenesis in GFAP-IκBα-dn following SCI. These studies suggest that therapeutic strategies targeting NF-kB activation in the CNS following SCI may promote oligogenesis and remyelination.
 
Overall design Wild type (WT) mice - time points naïve, 3 days, 3 weeks, 6 weeks. Transgenic mice (TG) - time points naïve, 3 days, 3 weeks, 6 weeks.
 
Contributor(s) Bracchi-Ricard V, Lambertsen KL, Ricard J, Nathanson L, Karmally S, Johnstone J, Ellman DG, Frydel B, McTigue D, Bethea JR
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Submission date May 07, 2013
Last update date May 10, 2018
Contact name John R. Bethea
E-mail(s) JBethea@med.miami.edu
Organization name University of Miami
Department The Miami Project to Cure Paralysis
Street address 1095 NW 14th Terrace
City Miami
State/province FL
ZIP/Postal code 33136
Country USA
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (22)
GSM1134638 WT3d repl1 vs WT naïve repl1
GSM1134639 WT3w repl2 vs WT3d repl2
GSM1134640 WT6w repl3 vs WT3d repl3
Relations
BioProject PRJNA202864

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Supplementary file Size Download File type/resource
GSE46695_RAW.tar 148.2 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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