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Series GSE4671 Query DataSets for GSE4671
Status Public on Jul 28, 2006
Title Microarray Analysis of the Delipidation of White Adipose Tissue of Mice Fed Conjugated Linoleic Acid
Organism Mus musculus
Experiment type Expression profiling by array
Summary The white adipose tissue (WAT) rapidly loses mass when mice are fed a diet containing trans-10, cis-12 conjugated linoleic acid (t10c12 CLA). A microarray analysis of WAT due to CLA feeding was performed to better define the processes and genes involved. WAT weight decreased by ca. 80% over 17 days of feeding a 0.5% t10c12 CLA diet. The lipid volume decreased by 90% and the number of adipocytes and total cells were reduced by15% and 47%, respectively. Microarray profiling of replicated pools of control and treated mice (n=140) at seven time points over the 17day feeding indicated between 2798 to 4318 genes showed mRNA changes of 2-fold or more. Transcript levels for genes of glucose and fatty acid import or biosynthesis were significantly reduced. A prolific inflammation response was indicated by the 2 to100-fold induction of many cytokine transcripts, including those for IL-6, IL1?, TNF ligands, and CXC family members
We used microarrays to characterize transcriptome changes in mouse adipose tissue due to feeding trans-10 cis-12 linoleic acid .
Keywords: time course
 
Overall design Mice were fed either diet with 0.5 % CLA or control diet, and the retroperitoneal fat pad mRNA surveyed by high density oligonucleotide array expression profiling. Microarray profiling of replicated pools of control and treated mice (n=140) at seven time points (day 1, 2, 3, 4, 10, 17) over the 17day feeding indicated between 2798 to 4318 genes showed mRNA changes of 2-fold or more.
 
Contributor(s) LaRosa CP, Miner J, Xia Y, Fromm ME, Kachman S
Citation(s) 16868072
Submission date Apr 13, 2006
Last update date Feb 11, 2019
Contact name Chris LaRosa
E-mail(s) plarosa1@unl.edu
Phone 4024724198
Fax 402472-3139
Organization name University of Nebraska, Lincoln
Department Center of Biotechnology
Street address 19th & Vine
City Lincoln
State/province NE
ZIP/Postal code 68588-0665
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (28)
GSM105363 retroperitoneal adipose tissue, Day 1, CLA treat
GSM105364 retroperitoneal adipose tissue, Day 1, CLA treat, Rep_2
GSM105365 retroperitoneal adipose tissue, Day 1, Control
Relations
BioProject PRJNA96771

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