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Series GSE4679 Query DataSets for GSE4679
Status Public on May 31, 2006
Title shRNAi induced differentiation time courses in mouse embrionic stem cells.
Organism Mus musculus
Experiment type Expression profiling by array
Summary We present an integrated approach to identify genetic mechanisms that control self-renewal in mouse embryonic stem (ES) cells. Short hairpin RNA (shRNA) techniques are employed to down regulate a set of gene-products whose expression patterns suggest self-renewal regulatory functions. We focus on transcriptional regulators and identify seven molecules whose shRNA-mediated depletion induces differentiation, including four whose roles in self-renewal had not been demonstrated.
We analyze the expression profiles of embryonic stems (ES) cells transduced with individual shRNA vectors, maintained in the presence of LIF.
Keywords: time course
 
Overall design We analyze transcriptome dynamics after down-regulating each of the 8 self-renewal regulators identified in the current studies. These are Nanog, Oct4, Sox2, Esrrb, Tbx3, Tcl1, Mm.343880 and Dppa4. Gene specific shRNA transduced GFP+ cells were FACS purified daily during a seven day interval and used to interrogate Affymetrix microarrays. Empty H1P vector served as a reference.
 
Contributor(s) Ivanova NB, Dobrin R, Lu R, Kotenko I, Levorse J, DeCoste C, Schafer X, Lemischka IR
Citation(s) 16767105
Submission date Apr 17, 2006
Last update date Jan 08, 2019
Contact name Ihor Lemischka
Organization name Princeton University
Street address Washington Rd
City Princeton
State/province NJ
ZIP/Postal code 08540
Country USA
 
Platforms (2)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
GPL340 [MOE430B] Affymetrix Mouse Expression 430B Array
Samples (140)
GSM105490 d0 empty vector MOE430A
GSM105491 d0 empty vector MOE430B
GSM105492 d1 empty vector MOE430A
Relations
BioProject PRJNA96785

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