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Series GSE46791 Query DataSets for GSE46791
Status Public on Jun 09, 2013
Title An epigenetic signature of developmental potential in neural stem cells and early neurons [expression]
Organism Mus musculus
Experiment type Expression profiling by array
Summary A cardinal property of neural stem cells (NSCs) is their ability to adopt multiple fates upon differentiation. The epigenome is widely seen as a read-out of cellular potential and a manifestation of this can be seen in embryonic stem cells (ESCs), where promoters of many lineage-specific regulators are marked by a bivalent epigenetic signature comprising trimethylation of both lysine 4 and lysine 27 of histone H3 (H3K4me3 and H3K27me3, respectively). Bivalency has subsequently emerged as a powerful epigenetic indicator of stem cell potential. Here, we have interrogated the epigenome during differentiation of ESC-derived NSCs to immature GABAergic interneurons. We show that developmental transitions are accompanied by loss of bivalency at many promoters in line with their increasing developmental restriction from pluripotent ESC through multipotent NSC to committed GABAergic interneuron. At the NSC stage, the promoters of genes encoding many transcriptional regulators required for differentiation of multiple neuronal subtypes and neural crest appear to be bivalent, consistent with the broad developmental potential of NSCs. Upon differentiation to GABAergic neurons, all non-GABAergic promoters resolve to H3K27me3 monovalency, whereas GABAergic promoters resolve to H3K4me3 monovalency or retain bivalency. Importantly, many of these epigenetic changes occur prior to any corresponding changes in gene expression. Intriguingly, another group of gene promoters gain bivalency as NSCs differentiate toward neurons, the majority of which are associated with functions connected with maturation and establishment and maintenance of connectivity. These data show that bivalency provides a dynamic epigenetic signature of developmental potential in both NSCs and in early neurons.
Overall design Illumina Expresson BeadChip arrays (MouseRef-8v2.0) were used to assess gene expression changes in neural stem cells (n=5; derived from mouse embryonic stem cells) and their differentiated neuronal progeny (n=3; FACS-purified based on Tau-RedStar expression).
Contributor(s) Burney MJ, Johnson C, Wong K, Teng S, Beglopoulos V, Stanton LW, Williams BP, Bithell A, Buckley NJ
Citation(s) 23712654
Submission date May 09, 2013
Last update date Jun 14, 2018
Contact name Matt Burney
Organization name King's College London
Street address 125 Coldharbour Lane
City London
ZIP/Postal code SE5 9NU
Country United Kingdom
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM1138448 NSC_1
GSM1138449 NSC_2
GSM1138450 NSC_3
This SubSeries is part of SuperSeries:
GSE46793 An epigenetic signature of developmental potential in neural stem cells and early neurons
BioProject PRJNA202309

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE46791_RAW.tar 3.1 Mb (http)(custom) TAR
GSE46791_non_normalized.txt.gz 848.4 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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