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Series GSE46895 Query DataSets for GSE46895
Status Public on Apr 25, 2014
Title SIRT1 but not its increased expression is essential for lifespan extension in caloric restricted mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary The SIRT1 deacetylase is one of the best-studied potential mediators of some of the anti-aging effects of calorie restriction (CR); but its role in CR-dependent lifespan extension has not been demonstrated. We previously found that mice lacking both copies of SIRT1 displayed a shorter median lifespan than wild type mice on an ad libitum diet. Here we report that median lifespan extension in CR heterozygote SIRT1+/- mice was identical (51%) to that observed in wild type mice but SIRT1+/- mice displayed a higher frequency of some certain pathologies. Although larger studies in different genetic backgrounds are necessary , these results provide strong initial evidence for the requirement of SIRT1 for the anti-aging effects of CR, but suggest that its high expression is not required for CR-induced lifespan extension.
Key words: SIRT1, caloric restriction, lifespan, anti-aging
Overall design 2-5 month old male mice of 3 different genotypes (SIRT1+/+, SIRT1+/-, and SIRT1-/-) that had normal, reduced or no expression of SIRT1 were treated with either a 40% caloric restricted diet (CR) or an ad libitum diet (AL). 2-4 replicates of each experimental condition were used in the analysis.
Contributor(s) Mercken EM, Hu J, Krzysik-Walker S, Wei M, Li Y, McBurney MW, de Cabo R, Longo VD
Citation(s) 23941528
Submission date May 14, 2013
Last update date Jun 22, 2020
Contact name Supriyo De
Organization name NIA-IRP, NIH
Department Laboratory of Genetics and Genomics
Lab Computational Biology & Genomics Core
Street address 251 Bayview Blvd
City Baltimore
State/province Maryland
ZIP/Postal code 21224
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (18)
GSM1139917 Liver-Caloric Restriction- Wt-replcate 1
GSM1139918 Liver-Caloric Restriction- Wt-replcate 2
GSM1139919 Liver-Caloric Restriction- Homozygous knockout-replicate 1
BioProject PRJNA202976

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46895_RAW.tar 3.1 Mb (http)(custom) TAR
GSE46895_non-normalized.txt.gz 2.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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