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| Status |
Public on Apr 25, 2006 |
| Title |
Changes in gene expression in dermal fibroblasts following exposure to Et1 peptide |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To determine if aberrant activation of endothelin-1 (Et1) could lead to the dysregulation of many downstream genes, we exposed fibroblasts to exogenous ET1 peptide and assayed for transcriptional changes by microarray. Mouse dermal fibroblasts were treated with exogenous Et1 peptide for 24 hours. ET1 treatment resulted in significant expression changes — primarily downregulation — of a number of genes. In particular, Tgfβ2 and Tgfβ3 were among the downregulated genes, which in turn alter the expression status of their many target genes. These data suggest that the stable silencing of Et1 is important for the phenotypic stability of dermal fibroblasts, and perhaps many other cell types as well.
Keywords: endothelin-1; Et1; dermal fibroblast
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| Overall design |
Three separate biological replicates were derived for both control and treated samples. The primary dermal fibroblasts were derived by explant procedure from the skin of mouse pups aged 0-3 days. By passage 5, cells were split to two separate cultures-- one with 100nM synthetic Et1 peptide added to the medium (treated) and the other with nothing added (control). Cells were exposed to Et1 for 24 hrs, then treated and control populations were harvested for total RNA.
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| Contributor(s) |
Vallender TW, Lahn BT |
| Citation(s) |
16870175 |
| Submission date |
Apr 20, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Tammy Vallender |
| E-mail(s) |
tfw@uchicago.edu
|
| Phone |
7738344065
|
| Organization name |
University of Chicago
|
| Department |
Human Genetics
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| Lab |
Dr. Bruce Lahn
|
| Street address |
929 E 57th St W503V
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA96743 |
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