|
| Status |
Public on Apr 25, 2006 |
| Title |
Gene expression changes following demethylation by 5-aza-2'-deoxycytidine in primary mouse dermal fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Methylation profiling by array
|
| Summary |
DNA methylation can contribute to the stable transcriptional silencing of mammalian genes. Oftentimes, these genes are important developmental regulators, and their silencing in cell types where they are not supposed to be active is important for the phenotypic stability of the cells. To identify key developmental regulator genes whose expression in terminally differentiated cells may be inhibited by DNA methylation, mouse dermal fibroblasts were demethylated with 5-aza-2’-deoxycytidine, and changes in gene expression monitored by microarray analysis.
Keywords: 5-aza-2'-deoxycytidine; methylation; dermal fibroblast
|
| |
|
| Overall design |
Three biological replicates for both control and 5-aza-2'-deoxycytidine treatment were derived. Cells were primary mouse dermal fibroblasts derived by explant procedure separately for each biological replicate. Treated cells were exposed to 5uM 5-aza-2'-deoxycytidine for 96 hours with recovery in normal medium for 24 hours. Control cells were untreated.
|
| |
|
| Contributor(s) |
Vallender TW, Lahn BT |
| Citation(s) |
16870175 |
| Submission date |
Apr 20, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Tammy Vallender |
| E-mail(s) |
tfw@uchicago.edu
|
| Phone |
7738344065
|
| Organization name |
University of Chicago
|
| Department |
Human Genetics
|
| Lab |
Dr. Bruce Lahn
|
| Street address |
929 E 57th St W503V
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA96745 |
Less...
More...