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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 15, 2006 |
| Title |
Developmental changes in RNP and Polysome associated mRNAs in Mouse testes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gametes rely heavily on post-transcriptional control mechanisms to regulate their differentiation. In eggs, the storage and selective temporal activation of maternal mRNAs is essential for normal development. In the male, transcription ceases during spermiogenesis necessitating the post-transcriptional regulation of many paternal mRNAs required for spermatid differentiation and spermatozoan function. Messenger RNAs that are being actively translated form polysomes. whereas translationally inactive mRNAs are often sequestered in ribonucleoproteins (RNPs). Here we combine polysome display and microarray analyses of RNP and polysome fractions of testes from prepuberal and adult mice to characterize the translation state of individual mRNAs as spermatogenesis proceeds.. Consistent with published reports, many post-meiotic mRNAs known to be translationally delayed shift from the RNPs into the polysomes, confirming the validity of this approach. In addition, based upon the criterion of movement from RNPs to polysomes, we detect another 742 mouse testicular genes showing dramatic shifts between RNPs and polysomes. One sub-group of 35 genes including the known translationally delayed Pgk2, are initially transcribed and translationally repressed in meiotic spermatocytes, and translated post-meiotically. This high-through-put approach defines the changing translation patterns of a large number of genes as male germ cells differentiate and identifies a new group of post-transcriptionally regulated meiotic transcripts for future study.
Keywords: time course
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| Overall design |
Mouse testes from animals of 3 different ages were fractionated on a sucrose gradient and transcripts were quantified in the RNP and Polysome fractions. Transcripts were identified that changed their RNP/Polysome representation at the diffent developmental time points.
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| Contributor(s) |
Iguchi N, Tobias JW, Hecht NB |
| Citation(s) |
16682651 |
| Submission date |
Apr 24, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Norman B Hecht |
| Organization name |
University of Pennsylvania
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| Street address |
421 Curie Blvd
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (18)
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| GSM106408 |
Polysome fraction from 17day-old mouse testes, biological rep1 |
| GSM106409 |
Polysome fraction from 17day-old mouse testes, biological rep2 |
| GSM106410 |
Polysome fraction from 17day-old mouse testes, biological rep3 |
| GSM106411 |
RNP fraction from 17day-old mouse testes, biological rep1 |
| GSM106412 |
RNP fraction from 17day-old mouse testes, biological rep2 |
| GSM106413 |
RNP fraction from 17day-old mouse testes, biological rep3 |
| GSM106414 |
Polysome fraction from 22 day-old mouse testes, biological rep1 |
| GSM106415 |
Polysome fraction from 22 day-old mouse testes, biological rep2 |
| GSM106416 |
Polysome fraction from 22 day-old mouse testes, biological rep3 |
| GSM106417 |
RNP fraction from 22day-old mouse testes, biological rep1 |
| GSM106418 |
RNP fraction from 22day-old mouse testes, biological rep2 |
| GSM106419 |
RNP fraction from 22day-old mouse testes, biological rep3 |
| GSM106420 |
Polysome fraction from adult mouse testes, biological rep1 |
| GSM106421 |
Polysome fraction from adult mouse testes, biological rep2 |
| GSM106422 |
Polysome fraction from adult mouse testes, biological rep3 |
| GSM106423 |
RNP fraction fromadult mouse testes, biological rep1 |
| GSM106424 |
RNP fraction fromadult mouse testes, biological rep2 |
| GSM106425 |
RNP fraction fromadult mouse testes, biological rep3 |
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| Relations |
| BioProject |
PRJNA95595 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE4711_RAW.tar |
58.8 Mb |
(http)(custom) |
TAR (of CEL) |
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