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Series GSE47119 Query DataSets for GSE47119
Status Public on Jun 13, 2013
Title Genomic mapping of ERG, AR, histone H3 monomethyl-K4, histone H3 trimethyl-K4 binding sites in mouse prostates in WT, ERG overexpression, Pten loss mouse prostates
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Translocation of ETS transcription factors including ERG and ETV1 occur in half of all prostate cancers. We generated a mouse model of ERG ovexpression (Rosa26-ERG) which when crossed into prostate specific probasin-Cre, expressed ERG specifically in the prostate. We crossed Rosa26-ERG into Pten flox/flox allele to generate compound GEMM mouse. Here, we determined the genomic binding sites of ERG, AR, and the histone marks H3K4me1 and H3K4me3 that maps enhancers and promoters respectively in the prostates of these mice.
 
Overall design The prostate of four six month old mice of each genotype were pooled. The chromatin was isolated and ChIP-Seq performed.
 
Contributor(s) Chen Y, Sawyers CL
Citation(s) 23817021
Submission date May 20, 2013
Last update date May 15, 2019
Contact name Yu Chen
E-mail(s) cheny1@mskcc.org
Phone 646-888-2163
Organization name Memorial Sloan Kettering Cancer Center
Department Human Oncology and Pathogenesis Program
Lab Chen
Street address 1275 York Ave, Box 20
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (20)
GSM1145302 Mouse_ERG_AR_IP
GSM1145303 Mouse_ERG_ERG_IP
GSM1145304 Mouse_ERG_H3K4me1_IP
This SubSeries is part of SuperSeries:
GSE47220 ETS factors reprogram the androgen receptor cistrome and prime prostate tumorigenesis in response to PTEN loss
Relations
BioProject PRJNA203677
SRA SRP022923

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47119_RAW.tar 5.0 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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