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Series GSE4712 Query DataSets for GSE4712
Status Public on Oct 24, 2006
Title Edema Toxin-treated Macrophage Study
Organism Mus musculus
Experiment type Expression profiling by array
Summary Edema toxin (EdTx), which is a combination of edema factor and a binding moiety (protective antigen), is produced by Bacillus anthracis, the etiological agent of anthrax. EdTx is an adenylyl cyclase enzyme that converts adenosine triphosphate to adenosine-3’,5’-monophosphate, resulting in interstitial edema seen in anthrax patients. We used GeneChip analysis to examine global transcriptional profiles of EdTx-treated RAW 264.7 murine macrophage-like cells at 3 and 6 hr.
Keywords: Toxin response
Overall design RAW 264.7 cells were treated with EdTx (2.5 µg/ml of protective antigen and 0.625 µg/ml of Edema factor), PA (2.5 µg/ml), or LPS (1 ng/mL) for 0, 3, and 6 hr. Each experiment was performed in triplicate, generating a total of 21 arrays (biological replciates).
Contributor(s) Comer JE, Galindo CL, Zhang F, Wenglikowski AM, Bush KL, Peterson JW, Chopra AK
Citation(s) 16846716
Submission date Apr 24, 2006
Last update date Feb 11, 2019
Contact name Ashok K Chopra
Phone 409-747-0573
Fax 409-747-6869
Organization name University of Texas Medical Branch
Department Microbiology & Immunology
Street address 301 University Blvd.
City Galveston
State/province TX
ZIP/Postal code 77555
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (21)
GSM106386 EdTx study_macrophages_ET-treated_3h_rep1
GSM106387 EdTx study_macrophages_ET-treated_3h_rep2
GSM106388 EdTx study_macrophages_ET-treated_3h_rep3
BioProject PRJNA95597

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