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| Status |
Public on May 22, 2013 |
| Title |
Vector-transduced neurons transcriptome profiles |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
With the goal of specifically dissecting the toxicogenomic signatures of the helper-dependent (HD) human (HAd5) and canine (CAV-2) adenovirus, the VSV-G-pseudotyped SIN HIV-1 (LV) and the Adenoviral-associated vector 2/9 for human neurons (AAV2/9), we transduced a bona fide human neuronal system with HD-HAd5, HD-CAV-2, LV and AAV2/9, we analysed the transcriptional response of more than 47,000 transcripts using gene chips.
Chip data showed that HD-CAV-2 and LV vectors both activated the innate arm of the immune response, including Toll-like receptors and hyaluronan circuits. LV vector induced as well an IFN response. Moreover, HD-CAV-2 and LV vectors affected DNA damage pathways - at 5 days in opposite directions - suggesting a differential response of the p53 and ATM pathways to the vector genomes. As a general response to the vectors, human neurons activated pro-survival genes and neuron morphogenesis.
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| Overall design |
Total RNAs extracted from transduced hmNPCs cells at 2 h and 5 days post-infection with HD-HAd, HD-CAV-2, LV and AAV2/9 vectors were hybridizated on Affymetrix microarrays and paired pair wise comparisons were performed between the mock and vector transduced hmNPCs cells. Each condition was tested in three replicates.
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| Contributor(s) |
Piersanti S, Astrologo L, Licursi V, Costa R, Roncaglia E, Gennetier A, Ibanes S, Chillon M, Negri R, Tagliafico E, Kremer EJ, Saggio I |
| Citation(s) |
23922808 |
| Submission date |
May 21, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Valerio Licursi |
| E-mail(s) |
valerio.licursi@uniroma1.it
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| Organization name |
Institute of Molecular Biology and Pathology (IBPM), National Research Council (CNR)
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| Department |
c/o Dept. Biology and Biotechnologies "C. Darwin", Sapienza University of Rome
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| Street address |
via degli Apuli, 4
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| City |
Rome |
| ZIP/Postal code |
00185 |
| Country |
Italy |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA203697 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47130_RAW.tar |
139.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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