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| Status |
Public on May 22, 2013 |
| Title |
Adenovirus E1A oncogene induces re-replication of cellular DNA and alters DNA replication dynamics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human adenovirus type 5 oncogene E1A was shown to induce massive re-replication of cellular DNA in late S phase. Using DNA combing analysis, it was shown that DNA replication dynamics including replicon length, fork velocity and inter-origin distance were dramatically altered in E1A expressing cells as compared to that of normal serum stimulated cells. It was also shown that c-Myc that is induced in E1A expressing cells is essential for the efficient entry of E1A expressing cells into S phase.
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| Overall design |
Serum starved cells were infected with an adenovirus expressing only the transforming E1A protein, and with Adbeta-galactosidase (control) were analyzed by flow cytomery. DNA prepared from such cells were analyzed by DNA combing analysis and data obtained were examined statistically. Requirement of Myc in E1A induced S phase entry was also examined by using antisense Myc vector.
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| Contributor(s) |
Singhal G, Leo E, Krushna S, Setty G, Pommier Y, Thimmapaya B |
| Citation(s) |
23740993 |
| Submission date |
May 21, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Ghata Singhal |
| Phone |
312-503-5176
|
| Organization name |
Northwestern University
|
| Department |
Microbiology-Immunology
|
| Street address |
303 E Chicago Avenue
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (4)
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| GSM1145614 |
Quiescent MCF10A cells infected with Adb-gal B1 |
| GSM1145615 |
Quiescent MCF10A cells infected with Adb-gal B2 |
| GSM1145616 |
Quiescent MCF10A cells infected with AdE1A virus E1 |
| GSM1145617 |
Quiescent MCF10A cells infected with AdE1A virus E2 |
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| Relations |
| BioProject |
PRJNA204400 |
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