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Series GSE47153 Query DataSets for GSE47153
Status Public on Nov 04, 2013
Title Effect of propranolol on differential gene expression in peripheral blood monocytes from mice subject to repeated social defeat
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study tested the effects of repeated social defeat (RSD) on gene expression in peripheral blood monocytes and examined the extent to which these effects were abrogated by the beta-adrenergic antagonist propranolol.
Study Type: Risk prediction
 
Overall design Gene expression profiling was carried out on peripheral blood monocyte mRNA samples collected from 36 mice randomized to either 6 cycles of repeated social defeat (RSD, n=18) or to parallel home cage control (HCC, n=18) conditions. Within each condition (RSD vs HCC), 9 animals were treated with the beta-adrenergic antagonist propranolol and 9 were treated with an equivalent volume of vehicle. After 6 cycles of RSD or parallel HCC, blood samples were pooled into groups of n=3 samples in each condition, and Illumina Mouse Ref-8 BeadArray assays were performed on RNA from approximately 1 million CD11b+ peripheral blood mononuclear cells (i.e., monocytes) which were immunomagnetically isolated by MACS. The primary research questions are 1) whether expression of pro-inflammatory genes is altered by RSD, and 2) whether treatment with propranolol abrogates these effects.
 
Contributor(s) Cole S
Citation(s) 24062448
Submission date May 21, 2013
Last update date Jun 14, 2018
Contact name Steve Cole
Organization name UCLA School of Medicine
Department Medicine
Street address 11-934 Factor Bldg, UCLA
City Los Angeles
State/province CA
ZIP/Postal code 90095-1678
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM1145798 HCC Vehicle 1
GSM1145799 HCC Vehicle 2
GSM1145800 HCC Vehicle 3
Relations
BioProject PRJNA204592

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47153_BB-QuantileNormGeneData.txt.gz 902.0 Kb (ftp)(http) TXT
GSE47153_BB-RawProbeData.txt.gz 1.3 Mb (ftp)(http) TXT
GSE47153_RAW.tar 3.1 Mb (http)(custom) TAR
Raw data are available on Series record
Processed data are available on Series record

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