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| Status |
Public on Jan 21, 2014 |
| Title |
Tissue-specific pioneer factors associate with androgen receptor cistromes and transcription programs. [microarray] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We report the in vivo androgen receptor (AR) binding sites in murine prostate, epididymis and kidney in response to physiological androgen testosterone using ChIP-sequencing and gene expression profiling by microarray. From AR cistrome analysis, we identified tissue-specific collaborating factors i.e. FoxA1 in prostate, Hnf4a in kidney and AP2a in epididymis and validated by ChIP-seq. The ChIP experiments have been performed using antibodies specific to AR, FoxA1, Hnf4a, AP-2a and IgG non-specific antibody as a negative control.
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| Overall design |
Expression profiling by microarray of mouse androgen responsive tissues, prostate, kidney and epididymis castrated and treated with vehicle or testosterone for 3 days or 12 or 24 hours after single testosterone-injection.
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| |
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| Contributor(s) |
Pihlajamaa P, Sahu B, Lyly L, Aittomäki V, Hautaniemi S, Jänne OA |
| Citation(s) |
24451200, 24459135 |
| Submission date |
May 22, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Olli A. Jänne |
| E-mail(s) |
olli.janne@helsinki.fi
|
| Phone |
+358919125040
|
| Organization name |
University of Helsinki
|
| Department |
Inst. of Biomedicine/Physiology
|
| Lab |
Androgen Receptor Laboratory
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| Street address |
Haartmaninkatu-8
|
| City |
Helsinki |
| ZIP/Postal code |
FI-00014 |
| Country |
Finland |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (45)
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| This SubSeries is part of SuperSeries: |
| GSE47194 |
Tissue-specific pioneer factors associate with androgen receptor cistromes and transcription programs |
|
| Relations |
| BioProject |
PRJNA205241 |
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