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| Status |
Public on Dec 31, 2013 |
| Title |
A haploid gene-trap mutant collection enables functional genetics in human cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Recently, gene-trap mutagenesis in near haploid human cells has been used to disrupt genes and identify novel host-pathogen interactions and elucidate mechanisms of drug action. Building on this technology, we here report the generation of a human gene-trap mutant collection of individual clones, which currently covers over a quarter of the expressed genome.
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| Overall design |
Strand specific RNA-Seq of ribosomal RNA depleted total cellular RNA to measure transcript abundance and to detect fusion transcripts in KBM7 cells at standard culture conditions.
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| Contributor(s) |
Bürckstümmer T, Pauler FM, Nijman SM |
| Citation missing |
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| Submission date |
May 28, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Florian M Pauler |
| E-mail(s) |
florian.pauler@ist.ac.at
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| Phone |
+43 2243 9000-7434
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| Organization name |
IST Austria
|
| Lab |
Simon Hippenmeyer
|
| Street address |
Am Campus 1
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| City |
Klosterneuburg |
| ZIP/Postal code |
3400 |
| Country |
Austria |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA205554 |
| SRA |
SRP023233 |
