|
| Status |
Public on Jul 22, 2013 |
| Title |
H3K27me3 is maintained at a reduced level in Suz12(Bgal/Bgal) ESCs [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Suz12(Bgal/Bgal) ESCs express a truncated form of Suz12 fused to Beta-galactosidase. These cells maintain a reduced level of H3K27me3 despite this mutation to a core component of PRC2, unlike Eed-/- ESCs whose H3K27me3 is ablated.
|
| |
|
| Overall design |
Two ESC lines mutant in genes of core components of Polycomb Repressive Complex 2 were assessed for H3K27me3 by ChIP-seq, as compared to a wild type ESC line.
|
| |
|
| Contributor(s) |
Thornton SR, Boyer LA |
| Citation(s) |
23955559 |
| Submission date |
May 29, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Laurie A Boyer |
| E-mail(s) |
lboyer@mit.edu
|
| Phone |
617 324-3335
|
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Biology
|
| Lab |
Boyer
|
| Street address |
77 Massachusetts Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (3) |
|
| This SubSeries is part of SuperSeries: |
| GSE47485 |
H3K27me3 is maintained at a reduced level in Suz12(Bgal/Bgal) ESCs |
|
| Relations |
| BioProject |
PRJNA205856 |
| SRA |
SRP023268 |
