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Status |
Public on Nov 15, 2013 |
Title |
DNA methylation analysis of subcutaneous adipose tissue abdominal versus subcutenaous adipose tissue gluteal |
Organism |
Homo sapiens |
Experiment type |
Methylation profiling by genome tiling array
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Summary |
In humans, adipose tissue is distributed in subcutaneous abdominal and subcutaneous gluteal depots that comprise a variety of functional differences. Whereas energy storage in gluteal adipose tissue has been shown to mediate a protective effect, an increase of abdominal adipose tissue is associated with metabolic disorders. However, the molecular basis of depot-specific characteristics is not completely understood yet. Using array-based analyses of transcription profiles, we identified a specific set of genes that was differentially expressed between subcutaneous abdominal and gluteal adipose tissue. To investigate the role of epigenetic regulation in depot-specific gene expression, we additionally analyzed genome-wide DNA methylation patterns in abdominal and gluteal depots. By combining both data sets, we identified a highly significant set of depot-specifically expressed genes that appear to be epigenetically regulated. Interestingly, the majority of these genes form part of the homeobox gene family. Moreover, genes involved in fatty acid metabolism were also differentially expressed. Therefore we suppose that changes in gene expression profiles might account for depot-specific differences in lipid composition. Indeed, triglycerides and fatty acids of abdominal adipose tissue were more saturated compared to triglycerides and fatty acids in gluteal adipose tissue. Taken together, our results uncover clear differences between abdominal and gluteal adipose tissue on the gene expression and DNA methylation level as well as in fatty acid composition. Therefore, a detailed molecular characterization of adipose tissue depots will be essential to develop new treatment strategies for metabolic syndrome associated complications.
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Overall design |
DNA methylation profiles of abdominal adipose tissue (6 samples) and gluteal adipose tissue (6 samples) were generated using Infinium methylation 450K BeadChips from Illumina (Illumina, San Diego, USA).
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Contributor(s) |
Gehrke S, Winnefeld M, Hagemann S |
Citation(s) |
24340035 |
Submission date |
May 30, 2013 |
Last update date |
Mar 22, 2019 |
Contact name |
Sandra Gehrke |
E-mail(s) |
sandraandrea.gehrke@beiersdorf.com
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Organization name |
Beiersdorf AG
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Department |
Applied Skin Biology
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Lab |
Special & Aged Skin
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Street address |
Unnastraße 48
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City |
Hamburg |
ZIP/Postal code |
20245 |
Country |
Germany |
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Platforms (1) |
GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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Samples (12)
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GSM1151333 |
10 AP_genomic DNA from subcutaneous adipose tissue |
GSM1151334 |
10 GLM_genomic DNA from subcutaneous adipose tissue |
GSM1151335 |
17 AP_genomic DNA from subcutaneous adipose tissue |
GSM1151336 |
17 GLM_genomic DNA from subcutaneous adipose tissue |
GSM1151337 |
22 AP_genomic DNA from subcutaneous adipose tissue |
GSM1151338 |
22 GLL_genomic DNA from subcutaneous adipose tissue |
GSM1151339 |
23 AP_genomic DNA from subcutaneous adipose tissue |
GSM1151340 |
23 GLL_genomic DNA from subcutaneous adipose tissue |
GSM1151341 |
26 AP_genomic DNA from subcutaneous adipose tissue |
GSM1151342 |
26 GLM_genomic DNA from subcutaneous adipose tissue |
GSM1151343 |
16 AP_genomic DNA from subcutaneous adipose tissue |
GSM1151344 |
16 GRM_genomic DNA from subcutaneous adipose tissue |
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This SubSeries is part of SuperSeries: |
GSE47513 |
Gene expression and DNA methylation analysis of subcutaneous abdominal and gluteal adipose tissue |
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Relations |
BioProject |
PRJNA205942 |