|
| Status |
Public on Sep 16, 2015 |
| Title |
Identification of s-RNYs targets in bone marrow-derived macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Bone marrow-derived macrophages (BMDMs) were collected from 4 C57BL/6J males and transfected with siRNAs against the terminal loop of mouse RNYs and control. 48 hr after transfection, the total RNA was isolated by using RNAeasy kit (Qiagen) and mRNA profiling was performed on Agilent Sureprint whole genomemicroarrays. The normalized data were analyzed with Biometric Research Branch (BRB) array tools 3.8.0. Log2-transformed signal intensities were used to identify probes with at least 1.2 fold change of expression difference between BMDMs overexpressing s-RNYs and control, and with a statistically significant p-value (p<0.05).
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| |
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| Overall design |
One color design, corresponding to 2 conditions performed on 4 mice: siRNA against terminal loop of RNY versus Control (Ct) siRNA for a total of 8 samples.
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| |
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| Contributor(s) |
Trabucchi M, Mari B, Barbry P, Lebrigand K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 30, 2013 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
|
| Samples (8)
|
| GSM1151379 |
252800511556-5_siRNAs TL RNYs Replicate 4 |
| GSM1151380 |
252800511556-3_siRNAs TL RNYs Replicate 1 |
| GSM1151381 |
252800511556-4_siRNA Ct Replicate 1 |
| GSM1151382 |
252800511556-2_siRNA Ct Replicate 2 |
| GSM1151383 |
252800511556-1_siRNAs TL RNYs Replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE47609 |
Atherogenic Stimuli Trigger Ago2-hnRNP A1-Dependent Processing of RNYs Regulating Apoptosis and Inflammatory Response in Macrophages |
|
| Relations |
| BioProject |
PRJNA205944 |
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