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| Status |
Public on Jun 01, 2014 |
| Title |
Jarid2, a Polycomb member and target of miR-155, regulates the fates of Th17 and Treg cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Genome-wide analysis of Jarid2, Suz12, and c-Maf binding and H3K27me3 profiling in miR-155 KO and WT Th17 performed by ChIP-seq. We found that Jarid2 and c-Maf is differentially expressed in absence of miR-155 and they compete for binding to the Il22 promoter. We highlight targets of Jarid2 and Suz12 in miR-155 KO Th17 cells that are epigenetically silenced by increased H3K27me3 status. Furthermore, genome-wide analysis through Suz12 ChIP-exo in WT and Jarid2fl/fl;CD4cre Th17 reveals defects in PRC2 recruitment in abscence of Jarid2 that results in derepression of genes in Th17 cells. Thus, one main function of miR-155 is to curb epigenetic silencing by targeting Jarid2.
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| |
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| Overall design |
Examination of Jarid2, Suz12, c-Maf binding and H3K27me3 changes in miR-155 KO and WT Th17.
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| |
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| Contributor(s) |
Kilaru GK, Escobar TM, Muljo SA |
| Citation(s) |
24856900 |
| Submission date |
May 30, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Gokhul Krishna Kilaru |
| E-mail(s) |
gokhul.kilaru@utsouthwestern.edu
|
| Phone |
214-648-4158
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| Organization name |
University of Texas Southwestern Medical Center
|
| Department |
Department of Neuroscience
|
| Lab |
Joseph S. Takahashi
|
| Street address |
6000 Harry Hines Boulevard
|
| City |
Dallas |
| State/province |
TEXAS |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA205981 |
| SRA |
SRP023466 |
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