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| Status |
Public on Aug 15, 2014 |
| Title |
Gene expression changes in Apc-mutant mouse intestinal organoids with and without deleting the Prox1 transcription factor |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We isolated and selected intestinal adenoma organoids from villin-CreER; Apcflox/flox and villin-CreER; Apcflox/flox; Prox1flox/flox mice and added tamoxifen to induce the deletion of the Apc and Prox1 genes in the intestinal epitheliul ex vivo. Microarray experiments were carried out 7 days after the addition of tamoxifen.
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| Overall design |
Total RNA obtained from villin-CreER; Apcflox/flox and villin-CreER; Apcflox/flox; Prox1flox/flox organoids were compared 7 days after the addition of tamoxifen and 5 days after the selection for Apc-mutant organoids in the absence of the Wnt-agonist R-Spondin1.
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| Contributor(s) |
Wiener Z, Hogstrom J, Hyvonen V, Alitalo K |
| Citation(s) |
25242330 |
| Submission date |
May 31, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Zoltan Wiener |
| E-mail(s) |
zoltan.wiener@helsinki.fi
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| Organization name |
University of Helsinki
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| Department |
Molecular Cancer Biology Programme
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| Street address |
Haartmaninkatu 8
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| City |
Helsinki |
| ZIP/Postal code |
FI-00017 |
| Country |
Finland |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (14)
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| GSM1152531 |
Apc-Prox1 deleted, Series1, repl2 |
| GSM1152532 |
Apc-deleted, Series1, repl3 |
| GSM1152533 |
Apc-deleted, Series1, repl4 |
| GSM1152534 |
Apc-Prox1 deleted, Series1, repl3 |
| GSM1152535 |
Apc-Prox1 deleted, Series1, repl4 |
| GSM1152536 |
Apc-deleted, Series2, repl1 |
| GSM1152537 |
Apc-Prox1 deleted, Series2, repl1 |
| GSM1152538 |
Apc-deleted, Series2, repl2 |
| GSM1152539 |
Apc-Prox1 deleted, Series2, repl2 |
| GSM1152540 |
Apc-deleted, Series2, repl3 |
| GSM1152541 |
Apc-Prox1 deleted, Series2, repl3 |
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| Relations |
| BioProject |
PRJNA206081 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47568_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE47568_non-normalized_1.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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