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| Status |
Public on Aug 05, 2013 |
| Title |
Regulation of Gene Expression by Connective Tissue Growth Factor (CTGF) in Mesenchymal Stromal Cells (MSCs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
mRNA from bone marrow-derived MSCs stably expressing CTGF-specific shRNA (or empty vector control) was analyzed for differential gene expression. Significant differences were found in cell proliferation-related genes, especially genes related to the M phase of the cell cycle, which were down-regulated in CTGF-knockdown-MSCs compared to control MSCs.
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| Overall design |
Bone marrow-derived MSCs were stably transduced with lentivirus expressing CTGF-specific shRNA or an empty vector control. After antibiotic selection, total RNA was amplified and hybridized to Illumina HT12 version 3 human whole-genome arrays (Illumina, San Diego, CA).
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| Contributor(s) |
Battula VL, Wang Z, Ma W, Davis RE, Andreeff M |
| Citation(s) |
23741006 |
| Submission date |
May 31, 2013 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Wencai Ma |
| Organization name |
MD Anderson Cancer Center
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| Street address |
7455 Fannin st
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| City |
Houston |
| ZIP/Postal code |
77054 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA206186 |
